Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells

Kazuhiro Aiba, Alexei A. Sharov, Mark G. Carter, Chiara Foroni, Angelo L. Vescovi, Minoru S.H. Ko

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

To understand global features of gene expression changes during in vitro neural differentiation, we carried out the microarray analysis of embryonic stem cells (ESCs), embryonal carcinoma cells, and adult neural stem/progenitor (NS) cells. Expression profiling of ESCs during differentiation in monolayer culture revealed three distinct phases: undifferentiated ESCs, primitive ectoderm-like cells, and neural progenitor cells. Principal component (PC) analysis revealed that these cells were aligned on PC1 over the course of 6 days. This PC1 represents approximately 4,000 genes, the expression of which increased with neural commitment/differentiation. Furthermore, NS cells derived from adult brain and their differentiated cells were positioned along this PC axis further away from undifferentiated ESCs than embryonic stem-derived neural progenitors. We suggest that this PC1 defines a path to neural fate, providing a scale for the degree of commitment/differentiation.

Original languageEnglish
Pages (from-to)889-895
Number of pages7
JournalStem Cells
Volume24
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1
Externally publishedYes

Keywords

  • Embryonic stem cells
  • Microarray
  • Neural commitment
  • Neural differentiation
  • Principal component analysis

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells'. Together they form a unique fingerprint.

  • Cite this