We have previously demonstrated that PMN newly released from the bone marrow preferentially sequester in pulmonary capillaries (1). To test the hypothesis that PMN newly released from the bone marrow (PMNBrdU) are less deformable than their circulating counterparts, PMN were labeled in the bone marrow with BrdU (100mg/kg, i.v.) 24 hr before BM release was induced by either saline (control), granulocyte colony stimulating factor (G-CSF, 12.5μg/kg, s.c.), or lipopolysaccharidefLPS, 10μg/kg i.v.) administration. Both G-CSF and LPS cause a significant increase in circulating PMN, band cell and PMNBrdU counts within 8 hr (p<0.05). Blood leukocytes harvested at 8 hr were filtered through a polycarbonate filter (pore size of Sum) by continuous infusion pump. The ratio of PMNBrdU present before and after filtration was determined by immunocytochemistry. In control animals PMNBrdU ratio approached unity (99.3± 3.6%, n=4). but it decreased significantly in the G-CSF (73.3±3.3%, p<0.01, n=4) and LPS (51.5±6.6%, p<0.01, n=4) treated animals. These results suggest that PMN released during active bone marrow release are less deformable than their circulating counterparts that may account for their preferential sequestration in pulmonary capillaries.
|Publication status||Published - 1996 Dec 1|
ASJC Scopus subject areas
- Molecular Biology