Abstract
Platelet glycoprotein GPIaIIa is an adhesive protein that recognizes collagen. We have investigated polymerized albumin particles conjugated with recombinant GPIaIIa (rGPIaIIa-poly Alb) for their platelet-like function. To evaluate the feasibility of these particles to achieve the hemostatic process, we measured the deformability (Young's modulus and spring constant) and the adhesive force of the particles using atomic force microscopy, which can measure the mechanical properties of individual cells. Our results showed that the Young's modulus of these particles was 2.3-fold larger than that of natural platelets and 12-fold larger than that of human red blood cells. The Young's modulus of the particles may have been determined by the properties of the polymerized albumin particle, although the glycoprotein of the platelet surface also contributed to the higher modulus. Our results also showed that the adhesive force of the rGPIaIIa-poly Alb with the collagen ligand was 52% of that of natural platelets. These two mechanical properties (deformability and adhesive force) of cells or particles, such as rGPIaIIa-poly Alb, are important specifications for the optimum design of platelet substitutes.
| Original language | English |
|---|---|
| Pages (from-to) | 35-40 |
| Number of pages | 6 |
| Journal | Journal of Biorheology |
| Volume | 23 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
Fingerprint
Keywords
- Adhesive force
- Atomic force microscopy
- Deformability
- Glycoprotein GPIaIIa
- Platelet substitutes
- Thrombocytopenia
- Young's modulus
ASJC Scopus subject areas
- Mechanics of Materials
- Materials Science(all)
- Mechanical Engineering
Cite this
Deformability and adhesive force of artificial platelets measured by atomic force microscopy. / Wada, Toru; Okamura, Yosuke; Takeoka, Shinji; Sudo, Ryo; Ikeda, Yasuo; Tanishita, Kazuo.
In: Journal of Biorheology, Vol. 23, No. 1, 2009, p. 35-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deformability and adhesive force of artificial platelets measured by atomic force microscopy
AU - Wada, Toru
AU - Okamura, Yosuke
AU - Takeoka, Shinji
AU - Sudo, Ryo
AU - Ikeda, Yasuo
AU - Tanishita, Kazuo
PY - 2009
Y1 - 2009
N2 - Platelet glycoprotein GPIaIIa is an adhesive protein that recognizes collagen. We have investigated polymerized albumin particles conjugated with recombinant GPIaIIa (rGPIaIIa-poly Alb) for their platelet-like function. To evaluate the feasibility of these particles to achieve the hemostatic process, we measured the deformability (Young's modulus and spring constant) and the adhesive force of the particles using atomic force microscopy, which can measure the mechanical properties of individual cells. Our results showed that the Young's modulus of these particles was 2.3-fold larger than that of natural platelets and 12-fold larger than that of human red blood cells. The Young's modulus of the particles may have been determined by the properties of the polymerized albumin particle, although the glycoprotein of the platelet surface also contributed to the higher modulus. Our results also showed that the adhesive force of the rGPIaIIa-poly Alb with the collagen ligand was 52% of that of natural platelets. These two mechanical properties (deformability and adhesive force) of cells or particles, such as rGPIaIIa-poly Alb, are important specifications for the optimum design of platelet substitutes.
AB - Platelet glycoprotein GPIaIIa is an adhesive protein that recognizes collagen. We have investigated polymerized albumin particles conjugated with recombinant GPIaIIa (rGPIaIIa-poly Alb) for their platelet-like function. To evaluate the feasibility of these particles to achieve the hemostatic process, we measured the deformability (Young's modulus and spring constant) and the adhesive force of the particles using atomic force microscopy, which can measure the mechanical properties of individual cells. Our results showed that the Young's modulus of these particles was 2.3-fold larger than that of natural platelets and 12-fold larger than that of human red blood cells. The Young's modulus of the particles may have been determined by the properties of the polymerized albumin particle, although the glycoprotein of the platelet surface also contributed to the higher modulus. Our results also showed that the adhesive force of the rGPIaIIa-poly Alb with the collagen ligand was 52% of that of natural platelets. These two mechanical properties (deformability and adhesive force) of cells or particles, such as rGPIaIIa-poly Alb, are important specifications for the optimum design of platelet substitutes.
KW - Adhesive force
KW - Atomic force microscopy
KW - Deformability
KW - Glycoprotein GPIaIIa
KW - Platelet substitutes
KW - Thrombocytopenia
KW - Young's modulus
UR - http://www.scopus.com/inward/record.url?scp=78651552085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651552085&partnerID=8YFLogxK
U2 - 10.1007/s12573-009-0007-y
DO - 10.1007/s12573-009-0007-y
M3 - Article
VL - 23
SP - 35
EP - 40
JO - Journal of Biorheology
JF - Journal of Biorheology
SN - 1867-0466
IS - 1
ER -