Degradation of T-kininogen by cathepsin D and matrix metalloproteinases

Wataru Sakamoto; Katsutoshi Fujie; Masayuki Kaga; Hiroshi Handa; Katsuhiro Gotoh; Jun Nishihira; Jun Ichi Kishi; Taro Hayakawa; Yasunori Okada

doi:10.1016/0162-3109(95)00054-2

Degradation of T-kininogen by cathepsin D and matrix metalloproteinases

Wataru Sakamoto, Katsutoshi Fujie, Masayuki Kaga, Hiroshi Handa, Katsuhiro Gotoh, Jun Nishihira, Jun Ichi Kishi, Taro Hayakawa, Yasunori Okada

Department of Pathology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Cathepsin D, matrix metalloproteinase (MMP)-2, MMP-3 (stromelysin), and MMP-9 were isolated from rat granulomatous tissues, HT1080 human fibrosarcoma cells and rheumatoid synovial cell CM. At acidic conditions, cathepsin D cleaved T-kininogen into small peptides and released Met-T-kinin-Leu (kinin precursor), but failed to release kinin. MMP-3 cleaved T-kininogen into a 57 kDa fragment as measured by SDS-PAGE and Western blot analysis using anti-T-kininogen antiserum. On the other hand, no degradation of T-kininogen occurred during incubation with MMP-2 or MMP-9100/1) at pH 7.5 for 7 h.

Original language	English
Pages (from-to)	73-75
Number of pages	3
Journal	Immunopharmacology
Volume	32
Issue number	1-3
DOIs	https://doi.org/10.1016/0162-3109(95)00054-2
Publication status	Published - 1996 May

Keywords

Cathepsin D
Kinin
Matrix metalloproteinase
T-kininogen

ASJC Scopus subject areas

Pharmacology

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10.1016/0162-3109(95)00054-2

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title = "Degradation of T-kininogen by cathepsin D and matrix metalloproteinases",

abstract = "Cathepsin D, matrix metalloproteinase (MMP)-2, MMP-3 (stromelysin), and MMP-9 were isolated from rat granulomatous tissues, HT1080 human fibrosarcoma cells and rheumatoid synovial cell CM. At acidic conditions, cathepsin D cleaved T-kininogen into small peptides and released Met-T-kinin-Leu (kinin precursor), but failed to release kinin. MMP-3 cleaved T-kininogen into a 57 kDa fragment as measured by SDS-PAGE and Western blot analysis using anti-T-kininogen antiserum. On the other hand, no degradation of T-kininogen occurred during incubation with MMP-2 or MMP-9100/1) at pH 7.5 for 7 h.",

keywords = "Cathepsin D, Kinin, Matrix metalloproteinase, T-kininogen",

author = "Wataru Sakamoto and Katsutoshi Fujie and Masayuki Kaga and Hiroshi Handa and Katsuhiro Gotoh and Jun Nishihira and Kishi, {Jun Ichi} and Taro Hayakawa and Yasunori Okada",

year = "1996",

month = may,

doi = "10.1016/0162-3109(95)00054-2",

language = "English",

volume = "32",

pages = "73--75",

journal = "Immunopharmacology",

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publisher = "Elsevier BV",

number = "1-3",

}

TY - JOUR

T1 - Degradation of T-kininogen by cathepsin D and matrix metalloproteinases

AU - Sakamoto, Wataru

AU - Fujie, Katsutoshi

AU - Kaga, Masayuki

AU - Handa, Hiroshi

AU - Gotoh, Katsuhiro

AU - Nishihira, Jun

AU - Kishi, Jun Ichi

AU - Hayakawa, Taro

AU - Okada, Yasunori

PY - 1996/5

Y1 - 1996/5

N2 - Cathepsin D, matrix metalloproteinase (MMP)-2, MMP-3 (stromelysin), and MMP-9 were isolated from rat granulomatous tissues, HT1080 human fibrosarcoma cells and rheumatoid synovial cell CM. At acidic conditions, cathepsin D cleaved T-kininogen into small peptides and released Met-T-kinin-Leu (kinin precursor), but failed to release kinin. MMP-3 cleaved T-kininogen into a 57 kDa fragment as measured by SDS-PAGE and Western blot analysis using anti-T-kininogen antiserum. On the other hand, no degradation of T-kininogen occurred during incubation with MMP-2 or MMP-9100/1) at pH 7.5 for 7 h.

AB - Cathepsin D, matrix metalloproteinase (MMP)-2, MMP-3 (stromelysin), and MMP-9 were isolated from rat granulomatous tissues, HT1080 human fibrosarcoma cells and rheumatoid synovial cell CM. At acidic conditions, cathepsin D cleaved T-kininogen into small peptides and released Met-T-kinin-Leu (kinin precursor), but failed to release kinin. MMP-3 cleaved T-kininogen into a 57 kDa fragment as measured by SDS-PAGE and Western blot analysis using anti-T-kininogen antiserum. On the other hand, no degradation of T-kininogen occurred during incubation with MMP-2 or MMP-9100/1) at pH 7.5 for 7 h.

KW - Cathepsin D

KW - Kinin

KW - Matrix metalloproteinase

KW - T-kininogen

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U2 - 10.1016/0162-3109(95)00054-2

DO - 10.1016/0162-3109(95)00054-2

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C2 - 8796270

AN - SCOPUS:0029890538

SN - 0162-3109

VL - 32

SP - 73

EP - 75

JO - Immunopharmacology

JF - Immunopharmacology

IS - 1-3

ER -

Degradation of T-kininogen by cathepsin D and matrix metalloproteinases

Abstract

Keywords

ASJC Scopus subject areas

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