Degradation of T-kininogen by cathepsin D and matrix metalloproteinases

Wataru Sakamoto, Katsutoshi Fujie, Masayuki Kaga, Hiroshi Handa, Katsuhiro Gotoh, Jun Nishihira, Jun Ichi Kishi, Taro Hayakawa, Yasunori Okada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cathepsin D, matrix metalloproteinase (MMP)-2, MMP-3 (stromelysin), and MMP-9 were isolated from rat granulomatous tissues, HT1080 human fibrosarcoma cells and rheumatoid synovial cell CM. At acidic conditions, cathepsin D cleaved T-kininogen into small peptides and released Met-T-kinin-Leu (kinin precursor), but failed to release kinin. MMP-3 cleaved T-kininogen into a 57 kDa fragment as measured by SDS-PAGE and Western blot analysis using anti-T-kininogen antiserum. On the other hand, no degradation of T-kininogen occurred during incubation with MMP-2 or MMP-9100/1) at pH 7.5 for 7 h.

Original languageEnglish
Pages (from-to)73-75
Number of pages3
JournalImmunopharmacology
Volume32
Issue number1-3
DOIs
Publication statusPublished - 1996 May

Fingerprint

Kininogens
Cathepsin D
Matrix Metalloproteinases
Matrix Metalloproteinase 3
Kinins
Matrix Metalloproteinase 2
Matrix Metalloproteinase 1
Fibrosarcoma
Matrix Metalloproteinase 9
Immune Sera
Polyacrylamide Gel Electrophoresis
Western Blotting
Peptides

Keywords

  • Cathepsin D
  • Kinin
  • Matrix metalloproteinase
  • T-kininogen

ASJC Scopus subject areas

  • Pharmacology

Cite this

Sakamoto, W., Fujie, K., Kaga, M., Handa, H., Gotoh, K., Nishihira, J., ... Okada, Y. (1996). Degradation of T-kininogen by cathepsin D and matrix metalloproteinases. Immunopharmacology, 32(1-3), 73-75. https://doi.org/10.1016/0162-3109(95)00054-2

Degradation of T-kininogen by cathepsin D and matrix metalloproteinases. / Sakamoto, Wataru; Fujie, Katsutoshi; Kaga, Masayuki; Handa, Hiroshi; Gotoh, Katsuhiro; Nishihira, Jun; Kishi, Jun Ichi; Hayakawa, Taro; Okada, Yasunori.

In: Immunopharmacology, Vol. 32, No. 1-3, 05.1996, p. 73-75.

Research output: Contribution to journalArticle

Sakamoto, W, Fujie, K, Kaga, M, Handa, H, Gotoh, K, Nishihira, J, Kishi, JI, Hayakawa, T & Okada, Y 1996, 'Degradation of T-kininogen by cathepsin D and matrix metalloproteinases', Immunopharmacology, vol. 32, no. 1-3, pp. 73-75. https://doi.org/10.1016/0162-3109(95)00054-2
Sakamoto W, Fujie K, Kaga M, Handa H, Gotoh K, Nishihira J et al. Degradation of T-kininogen by cathepsin D and matrix metalloproteinases. Immunopharmacology. 1996 May;32(1-3):73-75. https://doi.org/10.1016/0162-3109(95)00054-2
Sakamoto, Wataru ; Fujie, Katsutoshi ; Kaga, Masayuki ; Handa, Hiroshi ; Gotoh, Katsuhiro ; Nishihira, Jun ; Kishi, Jun Ichi ; Hayakawa, Taro ; Okada, Yasunori. / Degradation of T-kininogen by cathepsin D and matrix metalloproteinases. In: Immunopharmacology. 1996 ; Vol. 32, No. 1-3. pp. 73-75.
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