Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells

Yasumasa Hara, Midori A. Arai, Kanae Sakai, Naoki Ishikawa, Tohru Gonoi, Takashi Yaguchi, Masami Ishibashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

Original languageEnglish
Pages (from-to)280-289
Number of pages10
JournalJournal of Natural Medicines
Volume72
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Keywords

  • Actinomycetes
  • Amide
  • Co-culture
  • Nocardia

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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