Delayed phosphorylation of p38 mitogen-activated protein kinase in the AT1a knock-out mouse striatal neurons during middle cerebral artery occlusion and reperfusion

Hidenori Hattori, Mamoru Shibata, Takeshi Sugaya, Junichi Hamada, Yasuo Fukuuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate whether the phosphorylation of p38 in cerebral ischemia occurs via angiotensin II receptor type 1a (AT1a), we examined the time course of phosphorylation of p38 and proline-rich tyrosine kinase 2 in AT1a knock-out mouse striatal neurons during middle cerebral artery occlusion (MCAO) and reperfusion. Phosphorylated-p38 was observed after 2 h and 5 h of reperfusion after 1 h of MCAO in C57/B6 mice and AT1a knock out mice, respectively. We demonstrated a delay of phosphorylation of p38 in the reperfusion model of the AT1a knock-out mouse, and detected microglia in the striatum on the ischemic side that were phosphorylated-p38-positive after 71 h of reperfusion in both animals. However, there was no association between AT1a and delayed neuronal cell death, or between AT1a and activation of caspase-9 in cerebral ischemia/reperfusion.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalNeuroscience Letters
Volume341
Issue number1
DOIs
Publication statusPublished - 2003 Apr 24

Fingerprint

Corpus Striatum
Middle Cerebral Artery Infarction
p38 Mitogen-Activated Protein Kinases
Knockout Mice
Reperfusion
Phosphorylation
Neurons
Brain Ischemia
Focal Adhesion Kinase 2
Angiotensin Type 1 Receptor
Caspase 9
Microglia
Cell Death

Keywords

  • Angiotensin
  • Angiotensin II receptor type 1a
  • Cerebral ischemia and reperfusion
  • Delayed neuronal death
  • Knock-out mouse
  • Microglia
  • p38 mitogen-activated protein kinase
  • Proline-rich tyrosine kinase 2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Delayed phosphorylation of p38 mitogen-activated protein kinase in the AT1a knock-out mouse striatal neurons during middle cerebral artery occlusion and reperfusion. / Hattori, Hidenori; Shibata, Mamoru; Sugaya, Takeshi; Hamada, Junichi; Fukuuchi, Yasuo.

In: Neuroscience Letters, Vol. 341, No. 1, 24.04.2003, p. 9-12.

Research output: Contribution to journalArticle

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AU - Shibata, Mamoru

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AU - Hamada, Junichi

AU - Fukuuchi, Yasuo

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