Deletion mapping on chromosome 1p in well-differentiated gastric cancer

T. Ezaki, A. Yanagisawa, K. Ohta, S. Aiso, Mitsuhiro Watanabe, T. Hibi, Y. Kato, T. Nakajima, T. Ariyama, J. Inazawa, Y. Nakamura, A. Horii

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Abstract

To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands.

Original languageEnglish
Pages (from-to)424-428
Number of pages5
JournalBritish Journal of Cancer
Volume73
Issue number4
Publication statusPublished - 1996

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Chromosome Mapping
Tumor Suppressor Genes
Microsatellite Repeats
Stomach Neoplasms
Loss of Heterozygosity
Chromosomes, Human, Pair 1
Fluorescence In Situ Hybridization
Restriction Fragment Length Polymorphisms
Neoplasms
Stomach
Adenocarcinoma

Keywords

  • Chromosome 1p
  • Human gastric cancer
  • LOH

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ezaki, T., Yanagisawa, A., Ohta, K., Aiso, S., Watanabe, M., Hibi, T., ... Horii, A. (1996). Deletion mapping on chromosome 1p in well-differentiated gastric cancer. British Journal of Cancer, 73(4), 424-428.

Deletion mapping on chromosome 1p in well-differentiated gastric cancer. / Ezaki, T.; Yanagisawa, A.; Ohta, K.; Aiso, S.; Watanabe, Mitsuhiro; Hibi, T.; Kato, Y.; Nakajima, T.; Ariyama, T.; Inazawa, J.; Nakamura, Y.; Horii, A.

In: British Journal of Cancer, Vol. 73, No. 4, 1996, p. 424-428.

Research output: Contribution to journalArticle

Ezaki, T, Yanagisawa, A, Ohta, K, Aiso, S, Watanabe, M, Hibi, T, Kato, Y, Nakajima, T, Ariyama, T, Inazawa, J, Nakamura, Y & Horii, A 1996, 'Deletion mapping on chromosome 1p in well-differentiated gastric cancer', British Journal of Cancer, vol. 73, no. 4, pp. 424-428.
Ezaki T, Yanagisawa A, Ohta K, Aiso S, Watanabe M, Hibi T et al. Deletion mapping on chromosome 1p in well-differentiated gastric cancer. British Journal of Cancer. 1996;73(4):424-428.
Ezaki, T. ; Yanagisawa, A. ; Ohta, K. ; Aiso, S. ; Watanabe, Mitsuhiro ; Hibi, T. ; Kato, Y. ; Nakajima, T. ; Ariyama, T. ; Inazawa, J. ; Nakamura, Y. ; Horii, A. / Deletion mapping on chromosome 1p in well-differentiated gastric cancer. In: British Journal of Cancer. 1996 ; Vol. 73, No. 4. pp. 424-428.
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