Delivery of photosensitizer to cells by the stress wave induced by a single nanosecond laser pulse

Makoto Ogura, Shunichi Sato, Mitsuhiro Terakawa, Hitoshi Wakisaka, Maki Uenoyama, Tomosumi Ikeda, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We demonstrated that porfimer sodium (Photofrin II) was delivered into cells by applying a single nanosecond pulsed laser-induced stress wave (LISW). Analysis with a confocal laser scanning microscope showed that Photofrin molecules were homogeneously distributed in the cytoplasm at a low fluence (0.25 J/cm2), while the molecules were accumulated in a specific site at a high fluence (0.50 J/cm2). We also observed the nucleus membrane permeabilization induced by the LISW.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume42
Issue number8 A
Publication statusPublished - 2003 Aug 1

Fingerprint

stress waves
Photosensitizers
Laser pulses
delivery
fluence
Molecules
cytoplasm
Lasers
pulses
cells
Pulsed lasers
lasers
molecules
pulsed lasers
Microscopes
microscopes
Sodium
sodium
membranes
Membranes

Keywords

  • Drug delivery
  • Laser-induced stress wave
  • Photodynamic therapy
  • Photofrin II
  • Photosensitizer
  • Q-switched Nd:YAG laser
  • Shock wave

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Delivery of photosensitizer to cells by the stress wave induced by a single nanosecond laser pulse. / Ogura, Makoto; Sato, Shunichi; Terakawa, Mitsuhiro; Wakisaka, Hitoshi; Uenoyama, Maki; Ikeda, Tomosumi; Ashida, Hiroshi; Obara, Minoru.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 42, No. 8 A, 01.08.2003.

Research output: Contribution to journalArticle

Ogura, Makoto ; Sato, Shunichi ; Terakawa, Mitsuhiro ; Wakisaka, Hitoshi ; Uenoyama, Maki ; Ikeda, Tomosumi ; Ashida, Hiroshi ; Obara, Minoru. / Delivery of photosensitizer to cells by the stress wave induced by a single nanosecond laser pulse. In: Japanese Journal of Applied Physics, Part 2: Letters. 2003 ; Vol. 42, No. 8 A.
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