Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene

Yoshiyuki Konishi, Yasushi Kobayashi, Toshihiko Kishimoto, Yasutaka Makino, Atsushi Miyawaki, Teiichi Furuichi, Hideyuki Okano, Katsuhiko Mikoshiba, Taka Aki Tamura

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -396 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E- box consensus sequence. Electrophoretic mobility shift assay revealed CNS- related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay end competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.

Original languageEnglish
Pages (from-to)476-484
Number of pages9
JournalJournal of Neurochemistry
Volume69
Issue number2
Publication statusPublished - 1997 Aug
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Electrophoretic Mobility Shift Assay
Assays
Demonstrations
Genes
E-Box Elements
Electrophoretic mobility
Chloramphenicol O-Acetyltransferase
Cell-Free System
Purkinje Cells
Consensus Sequence
Transcription
Neuroblastoma
Cerebellum
Hippocampus
Transcription Factors
Tissue
Gene Expression
Brain
Gene expression

Keywords

  • Basic helix-loop-helix factor
  • E-box
  • In vitro transcription
  • Neuronal gene expression
  • Promoter
  • Type 1 inositol 1,4,5-trisphosphate receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Konishi, Y., Kobayashi, Y., Kishimoto, T., Makino, Y., Miyawaki, A., Furuichi, T., ... Tamura, T. A. (1997). Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene. Journal of Neurochemistry, 69(2), 476-484.

Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene. / Konishi, Yoshiyuki; Kobayashi, Yasushi; Kishimoto, Toshihiko; Makino, Yasutaka; Miyawaki, Atsushi; Furuichi, Teiichi; Okano, Hideyuki; Mikoshiba, Katsuhiko; Tamura, Taka Aki.

In: Journal of Neurochemistry, Vol. 69, No. 2, 08.1997, p. 476-484.

Research output: Contribution to journalArticle

Konishi, Y, Kobayashi, Y, Kishimoto, T, Makino, Y, Miyawaki, A, Furuichi, T, Okano, H, Mikoshiba, K & Tamura, TA 1997, 'Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene', Journal of Neurochemistry, vol. 69, no. 2, pp. 476-484.
Konishi, Yoshiyuki ; Kobayashi, Yasushi ; Kishimoto, Toshihiko ; Makino, Yasutaka ; Miyawaki, Atsushi ; Furuichi, Teiichi ; Okano, Hideyuki ; Mikoshiba, Katsuhiko ; Tamura, Taka Aki. / Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene. In: Journal of Neurochemistry. 1997 ; Vol. 69, No. 2. pp. 476-484.
@article{59a411c29c41414ebbdf03d4986fa467,
title = "Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene",
abstract = "The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -396 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E- box consensus sequence. Electrophoretic mobility shift assay revealed CNS- related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay end competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.",
keywords = "Basic helix-loop-helix factor, E-box, In vitro transcription, Neuronal gene expression, Promoter, Type 1 inositol 1,4,5-trisphosphate receptor",
author = "Yoshiyuki Konishi and Yasushi Kobayashi and Toshihiko Kishimoto and Yasutaka Makino and Atsushi Miyawaki and Teiichi Furuichi and Hideyuki Okano and Katsuhiko Mikoshiba and Tamura, {Taka Aki}",
year = "1997",
month = "8",
language = "English",
volume = "69",
pages = "476--484",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene

AU - Konishi, Yoshiyuki

AU - Kobayashi, Yasushi

AU - Kishimoto, Toshihiko

AU - Makino, Yasutaka

AU - Miyawaki, Atsushi

AU - Furuichi, Teiichi

AU - Okano, Hideyuki

AU - Mikoshiba, Katsuhiko

AU - Tamura, Taka Aki

PY - 1997/8

Y1 - 1997/8

N2 - The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -396 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E- box consensus sequence. Electrophoretic mobility shift assay revealed CNS- related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay end competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.

AB - The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -396 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E- box consensus sequence. Electrophoretic mobility shift assay revealed CNS- related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay end competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.

KW - Basic helix-loop-helix factor

KW - E-box

KW - In vitro transcription

KW - Neuronal gene expression

KW - Promoter

KW - Type 1 inositol 1,4,5-trisphosphate receptor

UR - http://www.scopus.com/inward/record.url?scp=0030853387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030853387&partnerID=8YFLogxK

M3 - Article

VL - 69

SP - 476

EP - 484

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 2

ER -