Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa

Naoki Hosoe, Soichiro Miura, Chikako Watanabe, Yoshikazu Tsuzuki, Ryota Hokari, Tokushige Oyama, Yoichi Fujiyama, Hiroshi Nagata, Hiromasa Ishii

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small intestine were fluorescence labeled, and their adhesion to mucosal microvessels was observed by intravital microscopy. Lamina proprial lymphocytes (LPL) and intraepithelial lymphocytes (IEL) adhered to both the small intestine and colon, and desensitization of CCR9 with TECK/CCL25 or anti-TECK/CCL25 antibody significantly inhibited these adhesions only in small intestine. At both sites, TNF-α significantly increased LPL adhesion but not IEL adhesion. Desensitization of CCR9 or anti-TECK/CCL25 antibody also attenuated the TNF-α-induced LPL adhesion in the small intestine. Increased expression of TECK/CCL25 by TNF-α was observed in the lamina propria of small intestine. TECK/CCL25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume286
Issue number3 49-3
Publication statusPublished - 2004 Mar

Fingerprint

Intestinal Mucosa
Chemokines
Thymus Gland
Endothelium
Small Intestine
Lymphocytes
T-Lymphocytes
Microvessels
Colon
CC Chemokines
Antibodies
Mucous Membrane
Fluorescence
Ligands

Keywords

  • CCR9
  • Intraepithelial lymphocyte
  • Intravital microscopy
  • Lamina proprial lymphocyte
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa. / Hosoe, Naoki; Miura, Soichiro; Watanabe, Chikako; Tsuzuki, Yoshikazu; Hokari, Ryota; Oyama, Tokushige; Fujiyama, Yoichi; Nagata, Hiroshi; Ishii, Hiromasa.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 286, No. 3 49-3, 03.2004.

Research output: Contribution to journalArticle

Hosoe, Naoki ; Miura, Soichiro ; Watanabe, Chikako ; Tsuzuki, Yoshikazu ; Hokari, Ryota ; Oyama, Tokushige ; Fujiyama, Yoichi ; Nagata, Hiroshi ; Ishii, Hiromasa. / Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2004 ; Vol. 286, No. 3 49-3.
@article{012b3d1fb1094f3d8555a1ee101e4b56,
title = "Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa",
abstract = "It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small intestine were fluorescence labeled, and their adhesion to mucosal microvessels was observed by intravital microscopy. Lamina proprial lymphocytes (LPL) and intraepithelial lymphocytes (IEL) adhered to both the small intestine and colon, and desensitization of CCR9 with TECK/CCL25 or anti-TECK/CCL25 antibody significantly inhibited these adhesions only in small intestine. At both sites, TNF-α significantly increased LPL adhesion but not IEL adhesion. Desensitization of CCR9 or anti-TECK/CCL25 antibody also attenuated the TNF-α-induced LPL adhesion in the small intestine. Increased expression of TECK/CCL25 by TNF-α was observed in the lamina propria of small intestine. TECK/CCL25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions.",
keywords = "CCR9, Intraepithelial lymphocyte, Intravital microscopy, Lamina proprial lymphocyte, Tumor necrosis factor-α",
author = "Naoki Hosoe and Soichiro Miura and Chikako Watanabe and Yoshikazu Tsuzuki and Ryota Hokari and Tokushige Oyama and Yoichi Fujiyama and Hiroshi Nagata and Hiromasa Ishii",
year = "2004",
month = "3",
language = "English",
volume = "286",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 49-3",

}

TY - JOUR

T1 - Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa

AU - Hosoe, Naoki

AU - Miura, Soichiro

AU - Watanabe, Chikako

AU - Tsuzuki, Yoshikazu

AU - Hokari, Ryota

AU - Oyama, Tokushige

AU - Fujiyama, Yoichi

AU - Nagata, Hiroshi

AU - Ishii, Hiromasa

PY - 2004/3

Y1 - 2004/3

N2 - It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small intestine were fluorescence labeled, and their adhesion to mucosal microvessels was observed by intravital microscopy. Lamina proprial lymphocytes (LPL) and intraepithelial lymphocytes (IEL) adhered to both the small intestine and colon, and desensitization of CCR9 with TECK/CCL25 or anti-TECK/CCL25 antibody significantly inhibited these adhesions only in small intestine. At both sites, TNF-α significantly increased LPL adhesion but not IEL adhesion. Desensitization of CCR9 or anti-TECK/CCL25 antibody also attenuated the TNF-α-induced LPL adhesion in the small intestine. Increased expression of TECK/CCL25 by TNF-α was observed in the lamina propria of small intestine. TECK/CCL25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions.

AB - It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small intestine were fluorescence labeled, and their adhesion to mucosal microvessels was observed by intravital microscopy. Lamina proprial lymphocytes (LPL) and intraepithelial lymphocytes (IEL) adhered to both the small intestine and colon, and desensitization of CCR9 with TECK/CCL25 or anti-TECK/CCL25 antibody significantly inhibited these adhesions only in small intestine. At both sites, TNF-α significantly increased LPL adhesion but not IEL adhesion. Desensitization of CCR9 or anti-TECK/CCL25 antibody also attenuated the TNF-α-induced LPL adhesion in the small intestine. Increased expression of TECK/CCL25 by TNF-α was observed in the lamina propria of small intestine. TECK/CCL25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions.

KW - CCR9

KW - Intraepithelial lymphocyte

KW - Intravital microscopy

KW - Lamina proprial lymphocyte

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=1342301689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342301689&partnerID=8YFLogxK

M3 - Article

VL - 286

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 3 49-3

ER -