Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice

Tango Handa, Takanori Kanai, Toshiro Sato, Yohei Mikami, Tomohisa Sujino, Atsushi Hayashi, Shinta Mizuno, Atsuhiro Matsumoto, Tadakazu Hisamatsu, Toshifumi Hibi

Research output: Contribution to journalArticle

Abstract

Intestinal lamina propria dendritic cells (LPDCs) in mice are known to extend dendrites between the intestinal epithelia and the luminal side when processing luminal antigens. We conducted intrarectal cell transfer experiments of bone marrow-derived dendritic cells (BMDCs) in mice to assess dendritic cell penetration of the intestine. Intrarectally administered GFP+ BMDCs localized in the colonic LP within 3h and the spleen within 12h after administration. 72h after administration, recipient C57BL/6 mice showed acute diarrhea, and administration of BMDCs (once weekly for 3 weeks) induced intestinal inflammation with increased numbers of recipient macrophages and CD4+ T cells exhibiting a Th2-mediated immune response. These results demonstrate that DCs actively communicate across the intestinal barrier, and highlight a potential technique for controlling colonic immune tolerance.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalImmunology Letters
Volume150
Issue number1-2
DOIs
Publication statusPublished - 2013 Feb

Fingerprint

Colitis
Dendritic Cells
Bone Marrow
Immune Tolerance
Antigen Presentation
Intestinal Mucosa
Dendrites
Inbred C57BL Mouse
Intestines
Diarrhea
Mucous Membrane
Spleen
Macrophages
Inflammation
T-Lymphocytes

Keywords

  • Dendritic cells
  • Intestinal tolerance
  • Rectal administration
  • Th2 mediated colitis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice. / Handa, Tango; Kanai, Takanori; Sato, Toshiro; Mikami, Yohei; Sujino, Tomohisa; Hayashi, Atsushi; Mizuno, Shinta; Matsumoto, Atsuhiro; Hisamatsu, Tadakazu; Hibi, Toshifumi.

In: Immunology Letters, Vol. 150, No. 1-2, 02.2013, p. 123-129.

Research output: Contribution to journalArticle

Handa, Tango ; Kanai, Takanori ; Sato, Toshiro ; Mikami, Yohei ; Sujino, Tomohisa ; Hayashi, Atsushi ; Mizuno, Shinta ; Matsumoto, Atsuhiro ; Hisamatsu, Tadakazu ; Hibi, Toshifumi. / Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice. In: Immunology Letters. 2013 ; Vol. 150, No. 1-2. pp. 123-129.
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