TY - JOUR
T1 - Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice
AU - Handa, Tango
AU - Kanai, Takanori
AU - Sato, Toshiro
AU - Mikami, Yohei
AU - Sujino, Tomohisa
AU - Hayashi, Atsushi
AU - Mizuno, Shinta
AU - Matsumoto, Atsuhiro
AU - Hisamatsu, Tadakazu
AU - Hibi, Toshifumi
PY - 2013/2/1
Y1 - 2013/2/1
N2 - Intestinal lamina propria dendritic cells (LPDCs) in mice are known to extend dendrites between the intestinal epithelia and the luminal side when processing luminal antigens. We conducted intrarectal cell transfer experiments of bone marrow-derived dendritic cells (BMDCs) in mice to assess dendritic cell penetration of the intestine. Intrarectally administered GFP+ BMDCs localized in the colonic LP within 3h and the spleen within 12h after administration. 72h after administration, recipient C57BL/6 mice showed acute diarrhea, and administration of BMDCs (once weekly for 3 weeks) induced intestinal inflammation with increased numbers of recipient macrophages and CD4+ T cells exhibiting a Th2-mediated immune response. These results demonstrate that DCs actively communicate across the intestinal barrier, and highlight a potential technique for controlling colonic immune tolerance.
AB - Intestinal lamina propria dendritic cells (LPDCs) in mice are known to extend dendrites between the intestinal epithelia and the luminal side when processing luminal antigens. We conducted intrarectal cell transfer experiments of bone marrow-derived dendritic cells (BMDCs) in mice to assess dendritic cell penetration of the intestine. Intrarectally administered GFP+ BMDCs localized in the colonic LP within 3h and the spleen within 12h after administration. 72h after administration, recipient C57BL/6 mice showed acute diarrhea, and administration of BMDCs (once weekly for 3 weeks) induced intestinal inflammation with increased numbers of recipient macrophages and CD4+ T cells exhibiting a Th2-mediated immune response. These results demonstrate that DCs actively communicate across the intestinal barrier, and highlight a potential technique for controlling colonic immune tolerance.
KW - Dendritic cells
KW - Intestinal tolerance
KW - Rectal administration
KW - Th2 mediated colitis
UR - http://www.scopus.com/inward/record.url?scp=84874526104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874526104&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2013.01.010
DO - 10.1016/j.imlet.2013.01.010
M3 - Article
C2 - 23333807
AN - SCOPUS:84874526104
VL - 150
SP - 123
EP - 129
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 1-2
ER -