Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma

Gen Shiihashi, Daisuke Ito, Itaru Arai, Yuki Kobayashi, Kanehiro Hayashi, Shintaro Otsuka, Kazunori Nakajima, Michisuke Yuzaki, Shigeyoshi Itohara, Norihiro Suzuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cytoplasmic aggregation of fused in sarcoma (FUS) is detected in brain regions affected by amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), which compose the disease spectrum, FUS proteinopathy. To understand the pathomechanism of ALS-FTD-associated FUS, we examined the behavior and cellular properties of an ALS mouse model overexpressing FUS with nuclear localization signal deletion. Mutant FUS transgenic mice showed hyperactivity, social interactional deficits, and impaired fear memory retrieval, all of which are compatible with FTD phenotypes. Histological analyses showed decreased dendritic spine and synaptic density in the frontal cortex before neuronal loss. Examination of cultured cells confirmed that mutant but not wild-type FUS was associated with decreased dendritic growth, mRNA levels, and protein synthesis in dendrites. These data suggest that cytoplasmic FUS aggregates impair dendritic mRNA trafficking and translation, in turn leading to dendritic homeostasis disruption and the development of FTD phenotypes.

Original languageEnglish
JournalEBioMedicine
DOIs
Publication statusAccepted/In press - 2017

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Frontotemporal Dementia
Sarcoma
Homeostasis
Messenger RNA
Brain
Agglomeration
Cells
Data storage equipment
Proteins
Phenotype
Nuclear Localization Signals
Dendritic Spines
Amyotrophic Lateral Sclerosis
Protein Biosynthesis
Frontal Lobe
Dendrites
Transgenic Mice
Fear
Cultured Cells
Growth

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma. / Shiihashi, Gen; Ito, Daisuke; Arai, Itaru; Kobayashi, Yuki; Hayashi, Kanehiro; Otsuka, Shintaro; Nakajima, Kazunori; Yuzaki, Michisuke; Itohara, Shigeyoshi; Suzuki, Norihiro.

In: EBioMedicine, 2017.

Research output: Contribution to journalArticle

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AU - Hayashi, Kanehiro

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AU - Nakajima, Kazunori

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AU - Suzuki, Norihiro

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