Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS

Yoshiaki Ohashi, Akiyoshi Hirayama, Takamasa Ishikawa, Seira Nakamura, Kaori Shimizu, Yuki Ueno, Masaru Tomita, Tomoyoshi Soga

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Metabolic changes in response to histidine starvation were observed in histidine-auxotrophic Escherichia coli using a capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)-based metabolomics technique. Prior to the analysis, we prepared an E. coli metabolome list of 727 metabolites reported in the literature. An improved method for metabolite extraction was developed, which resulted in higher extraction efficiency in phosphate-rich metabolites, e.g., ATP and GTP. Based on the results, 375 charged, hydrophilic intermediates in primary metabolisms were analysed simultaneously, providing quantitative data of 198 metabolites. We confirmed that the intracellular levels of intermediates in histidine biosynthesis are rapidly accumulated in response to a drop in histidine level under histidine-starved conditions. Simultaneously, disciplined responses were observed in the glycolysis, tricarboxylic acid cycle, and amino acid and nucleotide biosynthesis pathways as regulated by amino acid starvation. This journal is

Original languageEnglish
Pages (from-to)135-147
Number of pages13
JournalMolecular BioSystems
Volume4
Issue number2
DOIs
Publication statusPublished - 2008

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Capillary electrophoresis
Metabolome
Capillary Electrophoresis
Histidine
Escherichia coli
Mass spectrometry
Mass Spectrometry
Metabolites
Biosynthesis
Starvation
Amino Acids
Metabolomics
Citric Acid Cycle
Glycolysis
Guanosine Triphosphate
Metabolism
Nucleotides
Adenosine Triphosphate
Phosphates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Ohashi, Y., Hirayama, A., Ishikawa, T., Nakamura, S., Shimizu, K., Ueno, Y., ... Soga, T. (2008). Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. Molecular BioSystems, 4(2), 135-147. https://doi.org/10.1039/b714176a

Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. / Ohashi, Yoshiaki; Hirayama, Akiyoshi; Ishikawa, Takamasa; Nakamura, Seira; Shimizu, Kaori; Ueno, Yuki; Tomita, Masaru; Soga, Tomoyoshi.

In: Molecular BioSystems, Vol. 4, No. 2, 2008, p. 135-147.

Research output: Contribution to journalArticle

Ohashi, Y, Hirayama, A, Ishikawa, T, Nakamura, S, Shimizu, K, Ueno, Y, Tomita, M & Soga, T 2008, 'Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS', Molecular BioSystems, vol. 4, no. 2, pp. 135-147. https://doi.org/10.1039/b714176a
Ohashi Y, Hirayama A, Ishikawa T, Nakamura S, Shimizu K, Ueno Y et al. Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. Molecular BioSystems. 2008;4(2):135-147. https://doi.org/10.1039/b714176a
Ohashi, Yoshiaki ; Hirayama, Akiyoshi ; Ishikawa, Takamasa ; Nakamura, Seira ; Shimizu, Kaori ; Ueno, Yuki ; Tomita, Masaru ; Soga, Tomoyoshi. / Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS. In: Molecular BioSystems. 2008 ; Vol. 4, No. 2. pp. 135-147.
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