Derivation and morphological characterization of mouse spermatogonial stem cell lines

Takehiko Ogawa, Masako Ohmura, Yoichi Tamura, Kaoru Kita, Kazuyuki Ohbo, Toshio Suda, Yoshinobu Kubota

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalArchives of Histology and Cytology
Volume67
Issue number4
DOIs
Publication statusPublished - 2004 Nov

Fingerprint

Testis
Stem Cells
Cell Line
Germ Cells
Spermatogonia
Adult Stem Cells
Genetic Engineering
Cell Biology
Spermatozoa
Cultured Cells
Lasers
Transplantation
Research
In Vitro Techniques

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Ogawa, T., Ohmura, M., Tamura, Y., Kita, K., Ohbo, K., Suda, T., & Kubota, Y. (2004). Derivation and morphological characterization of mouse spermatogonial stem cell lines. Archives of Histology and Cytology, 67(4), 297-306. https://doi.org/10.1679/aohc.67.297

Derivation and morphological characterization of mouse spermatogonial stem cell lines. / Ogawa, Takehiko; Ohmura, Masako; Tamura, Yoichi; Kita, Kaoru; Ohbo, Kazuyuki; Suda, Toshio; Kubota, Yoshinobu.

In: Archives of Histology and Cytology, Vol. 67, No. 4, 11.2004, p. 297-306.

Research output: Contribution to journalArticle

Ogawa, T, Ohmura, M, Tamura, Y, Kita, K, Ohbo, K, Suda, T & Kubota, Y 2004, 'Derivation and morphological characterization of mouse spermatogonial stem cell lines', Archives of Histology and Cytology, vol. 67, no. 4, pp. 297-306. https://doi.org/10.1679/aohc.67.297
Ogawa, Takehiko ; Ohmura, Masako ; Tamura, Yoichi ; Kita, Kaoru ; Ohbo, Kazuyuki ; Suda, Toshio ; Kubota, Yoshinobu. / Derivation and morphological characterization of mouse spermatogonial stem cell lines. In: Archives of Histology and Cytology. 2004 ; Vol. 67, No. 4. pp. 297-306.
@article{2ae7bd52692b45aab5cfc681e752baf1,
title = "Derivation and morphological characterization of mouse spermatogonial stem cell lines",
abstract = "Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.",
author = "Takehiko Ogawa and Masako Ohmura and Yoichi Tamura and Kaoru Kita and Kazuyuki Ohbo and Toshio Suda and Yoshinobu Kubota",
year = "2004",
month = "11",
doi = "10.1679/aohc.67.297",
language = "English",
volume = "67",
pages = "297--306",
journal = "Archives of Histology and Cytology",
issn = "0914-9465",
publisher = "Japan Society of Histological Documentation",
number = "4",

}

TY - JOUR

T1 - Derivation and morphological characterization of mouse spermatogonial stem cell lines

AU - Ogawa, Takehiko

AU - Ohmura, Masako

AU - Tamura, Yoichi

AU - Kita, Kaoru

AU - Ohbo, Kazuyuki

AU - Suda, Toshio

AU - Kubota, Yoshinobu

PY - 2004/11

Y1 - 2004/11

N2 - Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.

AB - Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.

UR - http://www.scopus.com/inward/record.url?scp=13444256233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444256233&partnerID=8YFLogxK

U2 - 10.1679/aohc.67.297

DO - 10.1679/aohc.67.297

M3 - Article

VL - 67

SP - 297

EP - 306

JO - Archives of Histology and Cytology

JF - Archives of Histology and Cytology

SN - 0914-9465

IS - 4

ER -