Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma

Hideyuki Saya, Takashi Masuko, Yoshiyuki Hashimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fischer 344 (F344) rats hyperimmunized with syngeneic 9L R8 glioma cells produced antibody selective to glioma cells. Hybridomas prepared from the spleen cells of the immunized rat were cloned, and we obtained a hybridoma clone which produced monoclonal IgM antibody, termed FR77, that showed selectivity to glioma cells. Immunoperoxidase staining of cultured cells revealed that FR77 was reactive with 3 lines of rat glioma cells but not with normal F344 rat fibroblasts. Immuno-histochemical staining of F344 rat tissue sections with biotinylated FR77 demonstrated that FR77 could bind with glioma tissue developed by intracerebral injection of 9L R8 glioma cells but not to normal parts of the brain tissues and other normal tissues tested. The FR77-defined antigen was observed to be mainly localized in cytoplasm of glioma cells but a small portion of the antigen was also detected on the glioma cell surface.

Original languageEnglish
Pages (from-to)1198-1202
Number of pages5
JournalJapanese Journal of Cancer Research
Volume76
Issue number12
Publication statusPublished - 1985 Dec
Externally publishedYes

Fingerprint

Hybridomas
Brain Neoplasms
Glioma
Spleen
Monoclonal Antibodies
Inbred F344 Rats
Staining and Labeling
Antigens
Immunoglobulin M
Cultured Cells
Cytoplasm
Clone Cells
Fibroblasts
Injections
Antibodies
Brain

Keywords

  • Monoclonal rat antibody
  • Rat glioma
  • Syngeneic glioma immunization

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma. / Saya, Hideyuki; Masuko, Takashi; Hashimoto, Yoshiyuki.

In: Japanese Journal of Cancer Research, Vol. 76, No. 12, 12.1985, p. 1198-1202.

Research output: Contribution to journalArticle

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