Abstract
Fischer 344 (F344) rats hyperimmunized with syngeneic 9L R8 glioma cells produced antibody selective to glioma cells. Hybridomas prepared from the spleen cells of the immunized rat were cloned, and we obtained a hybridoma clone which produced monoclonal IgM antibody, termed FR77, that showed selectivity to glioma cells. Immunoperoxidase staining of cultured cells revealed that FR77 was reactive with 3 lines of rat glioma cells but not with normal F344 rat fibroblasts. Immuno-histochemical staining of F344 rat tissue sections with biotinylated FR77 demonstrated that FR77 could bind with glioma tissue developed by intracerebral injection of 9L R8 glioma cells but not to normal parts of the brain tissues and other normal tissues tested. The FR77-defined antigen was observed to be mainly localized in cytoplasm of glioma cells but a small portion of the antigen was also detected on the glioma cell surface.
| Original language | English |
|---|---|
| Pages (from-to) | 1198-1202 |
| Number of pages | 5 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 76 |
| Issue number | 12 |
| Publication status | Published - 1985 Dec |
| Externally published | Yes |
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Keywords
- Monoclonal rat antibody
- Rat glioma
- Syngeneic glioma immunization
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma. / Saya, Hideyuki; Masuko, Takashi; Hashimoto, Yoshiyuki.
In: Japanese Journal of Cancer Research, Vol. 76, No. 12, 12.1985, p. 1198-1202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma
AU - Saya, Hideyuki
AU - Masuko, Takashi
AU - Hashimoto, Yoshiyuki
PY - 1985/12
Y1 - 1985/12
N2 - Fischer 344 (F344) rats hyperimmunized with syngeneic 9L R8 glioma cells produced antibody selective to glioma cells. Hybridomas prepared from the spleen cells of the immunized rat were cloned, and we obtained a hybridoma clone which produced monoclonal IgM antibody, termed FR77, that showed selectivity to glioma cells. Immunoperoxidase staining of cultured cells revealed that FR77 was reactive with 3 lines of rat glioma cells but not with normal F344 rat fibroblasts. Immuno-histochemical staining of F344 rat tissue sections with biotinylated FR77 demonstrated that FR77 could bind with glioma tissue developed by intracerebral injection of 9L R8 glioma cells but not to normal parts of the brain tissues and other normal tissues tested. The FR77-defined antigen was observed to be mainly localized in cytoplasm of glioma cells but a small portion of the antigen was also detected on the glioma cell surface.
AB - Fischer 344 (F344) rats hyperimmunized with syngeneic 9L R8 glioma cells produced antibody selective to glioma cells. Hybridomas prepared from the spleen cells of the immunized rat were cloned, and we obtained a hybridoma clone which produced monoclonal IgM antibody, termed FR77, that showed selectivity to glioma cells. Immunoperoxidase staining of cultured cells revealed that FR77 was reactive with 3 lines of rat glioma cells but not with normal F344 rat fibroblasts. Immuno-histochemical staining of F344 rat tissue sections with biotinylated FR77 demonstrated that FR77 could bind with glioma tissue developed by intracerebral injection of 9L R8 glioma cells but not to normal parts of the brain tissues and other normal tissues tested. The FR77-defined antigen was observed to be mainly localized in cytoplasm of glioma cells but a small portion of the antigen was also detected on the glioma cell surface.
KW - Monoclonal rat antibody
KW - Rat glioma
KW - Syngeneic glioma immunization
UR - http://www.scopus.com/inward/record.url?scp=0022393979&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022393979&partnerID=8YFLogxK
M3 - Article
VL - 76
SP - 1198
EP - 1202
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 12
ER -