Dermal absorption of N,N-dimethylacetamide in human volunteers

T. Nomiyama, K. Omae, C. Ishizuka, T. Yamauchi, Y. Kawasumi, K. Yamada, H. Endoh, H. Sakurai

Research output: Contribution to journalArticle

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Abstract

Objectives: We investigated the potential for the dermal absorption of N,N-dimethylacetamide (DMAC: CAS No. 127-19-5) vapor, the biological half-life of N-methylacetamide (NMAC) in urine as the biological exposure item of DMAC, and the adjustment method for urinary concentrations. Methods: Twelve healthy male volunteers (mean age 25.2 years, range 21-43 years) were exposed to DMAC for 4 h on two occasions at intervals of 96 h or above. Each volunteer sat inside a whole-body-type exposure chamber for the dermal exposure experiment or outside the chamber for the inhalation exposure experiment. The temperature and relative humidity in the chamber were controlled at approximately 26°C and 40% in order to keep the skin (90% naked) of the volunteers dry. DMAC concentrations were 6.1 ± 1.3 ppm for dermal exposure and 6.1 ± 1.3 ppm for inhalation exposure. Urine samples were collected from 0 h through 36 h and at 48 h and 72 h after the exposure. Extrapolations from exposure concentrations for 4 h to 10 ppm for 8 h were performed. Results: Mean dermal absorption was estimated to be 40.4% of the total DMAC uptake. The biological half-lives of urinary NMAC were 9.0 ± 1.4 h and 5.6 ± 1.3 h via skin and lung, respectively. Mean NMAC in urine just after 5 consecutive workdays (8 h/day) at 10 ppm DMAC exposure was assumed to be 33.7 mg/g · Cr (18.6-70.0 mg/g · Cr). Creatinine-adjusted NMAC concentration in urine for each volunteer within 12 h after the exposure was more closely correlated with the total excretion amount of NMAC up to 36 h than with urinary-volume-adjusted or specific-gravity-adjusted NMAC concentration in both the dermal and inhalation exposure experiments. Conclusions: DMAC vapor was significantly absorbed through the skin. Estimated NMAC values indicate that 20 mg/g · Cr NMAC seems to be appropriate as the biological exposure index.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalInternational Archives of Occupational and Environmental Health
Volume73
Issue number2
Publication statusPublished - 2000 Mar

Fingerprint

Skin Absorption
Volunteers
Inhalation Exposure
Skin
Urine
Somatotypes
Specific Gravity
N-methylacetamide
dimethylacetamide
Humidity
Half-Life
Creatinine
Healthy Volunteers
Lung
Temperature

Keywords

  • Biological half-life
  • Biological monitoring
  • Dermal absorption
  • N,N-Dimethylacetamide
  • N-Methylformamide

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Nomiyama, T., Omae, K., Ishizuka, C., Yamauchi, T., Kawasumi, Y., Yamada, K., ... Sakurai, H. (2000). Dermal absorption of N,N-dimethylacetamide in human volunteers. International Archives of Occupational and Environmental Health, 73(2), 121-126.

Dermal absorption of N,N-dimethylacetamide in human volunteers. / Nomiyama, T.; Omae, K.; Ishizuka, C.; Yamauchi, T.; Kawasumi, Y.; Yamada, K.; Endoh, H.; Sakurai, H.

In: International Archives of Occupational and Environmental Health, Vol. 73, No. 2, 03.2000, p. 121-126.

Research output: Contribution to journalArticle

Nomiyama, T, Omae, K, Ishizuka, C, Yamauchi, T, Kawasumi, Y, Yamada, K, Endoh, H & Sakurai, H 2000, 'Dermal absorption of N,N-dimethylacetamide in human volunteers', International Archives of Occupational and Environmental Health, vol. 73, no. 2, pp. 121-126.
Nomiyama T, Omae K, Ishizuka C, Yamauchi T, Kawasumi Y, Yamada K et al. Dermal absorption of N,N-dimethylacetamide in human volunteers. International Archives of Occupational and Environmental Health. 2000 Mar;73(2):121-126.
Nomiyama, T. ; Omae, K. ; Ishizuka, C. ; Yamauchi, T. ; Kawasumi, Y. ; Yamada, K. ; Endoh, H. ; Sakurai, H. / Dermal absorption of N,N-dimethylacetamide in human volunteers. In: International Archives of Occupational and Environmental Health. 2000 ; Vol. 73, No. 2. pp. 121-126.
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abstract = "Objectives: We investigated the potential for the dermal absorption of N,N-dimethylacetamide (DMAC: CAS No. 127-19-5) vapor, the biological half-life of N-methylacetamide (NMAC) in urine as the biological exposure item of DMAC, and the adjustment method for urinary concentrations. Methods: Twelve healthy male volunteers (mean age 25.2 years, range 21-43 years) were exposed to DMAC for 4 h on two occasions at intervals of 96 h or above. Each volunteer sat inside a whole-body-type exposure chamber for the dermal exposure experiment or outside the chamber for the inhalation exposure experiment. The temperature and relative humidity in the chamber were controlled at approximately 26°C and 40{\%} in order to keep the skin (90{\%} naked) of the volunteers dry. DMAC concentrations were 6.1 ± 1.3 ppm for dermal exposure and 6.1 ± 1.3 ppm for inhalation exposure. Urine samples were collected from 0 h through 36 h and at 48 h and 72 h after the exposure. Extrapolations from exposure concentrations for 4 h to 10 ppm for 8 h were performed. Results: Mean dermal absorption was estimated to be 40.4{\%} of the total DMAC uptake. The biological half-lives of urinary NMAC were 9.0 ± 1.4 h and 5.6 ± 1.3 h via skin and lung, respectively. Mean NMAC in urine just after 5 consecutive workdays (8 h/day) at 10 ppm DMAC exposure was assumed to be 33.7 mg/g · Cr (18.6-70.0 mg/g · Cr). Creatinine-adjusted NMAC concentration in urine for each volunteer within 12 h after the exposure was more closely correlated with the total excretion amount of NMAC up to 36 h than with urinary-volume-adjusted or specific-gravity-adjusted NMAC concentration in both the dermal and inhalation exposure experiments. Conclusions: DMAC vapor was significantly absorbed through the skin. Estimated NMAC values indicate that 20 mg/g · Cr NMAC seems to be appropriate as the biological exposure index.",
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AU - Omae, K.

AU - Ishizuka, C.

AU - Yamauchi, T.

AU - Kawasumi, Y.

AU - Yamada, K.

AU - Endoh, H.

AU - Sakurai, H.

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N2 - Objectives: We investigated the potential for the dermal absorption of N,N-dimethylacetamide (DMAC: CAS No. 127-19-5) vapor, the biological half-life of N-methylacetamide (NMAC) in urine as the biological exposure item of DMAC, and the adjustment method for urinary concentrations. Methods: Twelve healthy male volunteers (mean age 25.2 years, range 21-43 years) were exposed to DMAC for 4 h on two occasions at intervals of 96 h or above. Each volunteer sat inside a whole-body-type exposure chamber for the dermal exposure experiment or outside the chamber for the inhalation exposure experiment. The temperature and relative humidity in the chamber were controlled at approximately 26°C and 40% in order to keep the skin (90% naked) of the volunteers dry. DMAC concentrations were 6.1 ± 1.3 ppm for dermal exposure and 6.1 ± 1.3 ppm for inhalation exposure. Urine samples were collected from 0 h through 36 h and at 48 h and 72 h after the exposure. Extrapolations from exposure concentrations for 4 h to 10 ppm for 8 h were performed. Results: Mean dermal absorption was estimated to be 40.4% of the total DMAC uptake. The biological half-lives of urinary NMAC were 9.0 ± 1.4 h and 5.6 ± 1.3 h via skin and lung, respectively. Mean NMAC in urine just after 5 consecutive workdays (8 h/day) at 10 ppm DMAC exposure was assumed to be 33.7 mg/g · Cr (18.6-70.0 mg/g · Cr). Creatinine-adjusted NMAC concentration in urine for each volunteer within 12 h after the exposure was more closely correlated with the total excretion amount of NMAC up to 36 h than with urinary-volume-adjusted or specific-gravity-adjusted NMAC concentration in both the dermal and inhalation exposure experiments. Conclusions: DMAC vapor was significantly absorbed through the skin. Estimated NMAC values indicate that 20 mg/g · Cr NMAC seems to be appropriate as the biological exposure index.

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