Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange

Yuichiro Koide, Yasuteru Urano, Akira Yatsushige, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

A novel design strategy for controlling the fluorescence and photosensitizing ability of thiazole orange (TO) has been developed. The validity of this approach was demonstrated by the synthesis of a 5-galactosidase-activatable photosensitizer, PhoTO-Gal, in which fluorescence is simultaneously activated. PhoTO-Gal was demonstrated to kill HEK293 lacZ(+) cells, which express 5-galactosidase, but not HEK293 lacZ(-) cells, under light illumination. Such activatable photosensitizers should allow more refined PDT without the side effect of prolonged light sensitivity and should also be useful as tools for reporter enzyme expression-specific cell ablation.

Original languageEnglish
Pages (from-to)6058-6059
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number17
DOIs
Publication statusPublished - 2009 May 6
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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