Design and Synthesis of an Activatable Photoacoustic Probe for Hypochlorous Acid

Takayuki Ikeno, Kenjiro Hanaoka, Shimpei Iwaki, Takuya Myochin, Yoshiaki Murayama, Hisashi Ohde, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Photoacoustic (PA) imaging is a novel imaging modality that combines the high contrast of optical imaging and the deep tissue penetration of ultrasound. PA imaging contrast agents targeting various biological phenomena have been reported, but the development of activatable PA probes, which show a PA signal only in the presence of target molecules, remains challenging in spite of their potential usefulness for real-time PA imaging of specific biomolecules in vivo. To establish a simple design strategy for activatable PA probes, we first designed and synthesized a silicon-rhodamine based near-infrared nonfluorescent dye, wsSiNQ660 (water-soluble SiNQ660), as a scaffold and demonstrated that it offers a high conversion efficiency from light to ultrasound compared to typical near-infrared fluorescent dyes. Importantly, absorption off/on strategies previously established for rhodamine-based fluorescent probes are also applicable to this nonfluorescent dye scaffold. We validated this approach by synthesizing an activatable PA probe for hypochlorous acid (HOCl) and confirmed that it enables three-dimensional imaging of HOCl in mouse subcutis.

Original languageEnglish
Pages (from-to)9086-9092
Number of pages7
JournalAnalytical Chemistry
Volume91
Issue number14
DOIs
Publication statusPublished - 2019 Jun 20
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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