Design and synthesis of an enzyme activity-based labeling molecule with fluorescence spectral change

Toru Komatsu, Kazuya Kikuchi, Hideo Takakusa, Kenjiro Hanaoka, Tasuku Ueno, Mako Kamiya, Yasuteru Urano, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

Methods of covalent labeling of a specific tag protein with small-molecular dyes play an important role in studying dynamic behaviors of proteins in living cells. On the basis of quinone methide chemistry, we designed and synthesized a β-galactosidase labeling probe, CMFβ-gal, which shows a fluorescence wavelength change accompanying the labeling reaction, owing to fluorescence resonance energy transfer (FRET). Since the FRET efficiency changes accompanying the labeling reaction, fluorescence of labeled protein can be observed separately from that of the unreacted probe, so immediate detection of the target protein is possible. This is the first report of a protein labeling probe which features a change of fluorescence wavelength upon reaction, allowing the labeled protein to be detected even in the presence of unreacted probe.

Original languageEnglish
Pages (from-to)15946-15947
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number50
DOIs
Publication statusPublished - 2006 Dec 20
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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