Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers

Kengo Hanaya, Yoshiyuki Kageyama, Masanori Kitamura, Shin Aoki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.

Original languageEnglish
Pages (from-to)1601-1615
Number of pages15
JournalHeterocycles
Volume82
Issue number2
DOIs
Publication statusPublished - 2010 Feb 28
Externally publishedYes

Fingerprint

Dopamine
Immunoglobulin G
Biotin
Complexation
Anti-Idiotypic Antibodies
Western Blotting
Enzyme-Linked Immunosorbent Assay
Recovery
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Pharmacology

Cite this

Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers. / Hanaya, Kengo; Kageyama, Yoshiyuki; Kitamura, Masanori; Aoki, Shin.

In: Heterocycles, Vol. 82, No. 2, 28.02.2010, p. 1601-1615.

Research output: Contribution to journalArticle

Hanaya, Kengo ; Kageyama, Yoshiyuki ; Kitamura, Masanori ; Aoki, Shin. / Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers. In: Heterocycles. 2010 ; Vol. 82, No. 2. pp. 1601-1615.
@article{9e17d550ee884cbeb4579ea1c5bd503c,
title = "Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers",
abstract = "Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.",
author = "Kengo Hanaya and Yoshiyuki Kageyama and Masanori Kitamura and Shin Aoki",
year = "2010",
month = "2",
day = "28",
doi = "10.3987/COM-10-S(E)124",
language = "English",
volume = "82",
pages = "1601--1615",
journal = "Heterocycles",
issn = "0385-5414",
publisher = "Japan Institute of Heterocyclic Chemistry",
number = "2",

}

TY - JOUR

T1 - Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers

AU - Hanaya, Kengo

AU - Kageyama, Yoshiyuki

AU - Kitamura, Masanori

AU - Aoki, Shin

PY - 2010/2/28

Y1 - 2010/2/28

N2 - Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.

AB - Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.

UR - http://www.scopus.com/inward/record.url?scp=79952756712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952756712&partnerID=8YFLogxK

U2 - 10.3987/COM-10-S(E)124

DO - 10.3987/COM-10-S(E)124

M3 - Article

AN - SCOPUS:79952756712

VL - 82

SP - 1601

EP - 1615

JO - Heterocycles

JF - Heterocycles

SN - 0385-5414

IS - 2

ER -