Design and synthesis of photocleavable biotinylated-dopamine with polyethyleneoxy photocleavable linkers

Kengo Hanaya, Yoshiyuki Kageyama, Masanori Kitamura, Shin Aoki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previously, we reported the synthesis of a biotin-dopamine conjugate (Btn-DA) with a photocleavable 8-quinolinyl benzenesulfonate (QB) linker for the isolation of an intact complex of DA and anti-DA antibody (IgG1) (DA-IgG1 complex). In this work, we synthesized a photocleavable Btn-DA conjugate with a polyethyleneoxy linker to improve the complexation efficiency and the recovery yields of DA-IgG1 complex. The results of QCM, ELISA, and Western blot analyses indicate that the introduction of polyethyleneoxy linkers improved the efficiency of DA-IgG1 complexation.

Original languageEnglish
Pages (from-to)1601-1615
Number of pages15
JournalHeterocycles
Volume82
Issue number2
DOIs
Publication statusPublished - 2010 Feb 28
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry

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