Design of Environmentally Responsive Fluorescent Polymer Probes for Cellular Imaging

Arisa Yamada, Yuki Hiruta, Jian Wang, Eri Ayano, Hideko Kanazawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We report the development of environmentally responsive fluorescent polymers. The reversible temperature-induced phase transition of copolymers composed of N-isopropylacrylamide and a fluorescent monomer based on the fluorescein (FL), coumarin (CO), rhodamine (RH), or dansyl (DA) skeleton was used as a molecular switch to control the fluorescence intensity. The poly(N-isopropylacrylamide) (PNIPAAm) chain showed an expanded coil conformation below the lower critical solution temperature (LCST) due to hydration, but it changed to a globular form above the LCST due to dehydration. Through the combination of a polarity-sensitive fluorophore with PNIPAAm, the synthetic fluorescent polymer displayed a response to external temperature, with the fluorescence strength dramatically changing close to the LCST. Additionally, the P(NIPAAm-co-FL) and P(NIPAAm-co-CO) polymers, containing fluorescein and coumarin groups, respectively, exhibited pH responsiveness. The environmental responsiveness of the reported polymers is derived directly from the PNIPAAm and fluorophore structures, thus allowing for the cellular uptake of the fluorescence copolymer by RAW264.7 cells to be temperature-controlled. Cellular uptake was suppressed below the LCST but enhanced above the LCST. Furthermore, the cellular uptake of both P(NIPAAm-co-CO) and P(NIPAAm-co-RH) conjugated with a fusogenic lipid, namely, L-α-phosphatidylethanolamine, dioleoyl (DOPE), was enhanced. Such lipid-conjugated fluorescence probes are expected to be useful as physiological indicators for intracellular imaging. (Chemical Equation Presented).

Original languageEnglish
Pages (from-to)2356-2362
Number of pages7
JournalBiomacromolecules
Volume16
Issue number8
DOIs
Publication statusPublished - 2015 Aug 10

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Polymers
Imaging techniques
Fluorescein
Fluorescence
Temperature
Rhodamines
Fluorophores
Lipids
Copolymers
Dehydration
Hydration
Conformations
Monomers
Phase transitions
Switches
coumarin
poly-N-isopropylacrylamide

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Design of Environmentally Responsive Fluorescent Polymer Probes for Cellular Imaging. / Yamada, Arisa; Hiruta, Yuki; Wang, Jian; Ayano, Eri; Kanazawa, Hideko.

In: Biomacromolecules, Vol. 16, No. 8, 10.08.2015, p. 2356-2362.

Research output: Contribution to journalArticle

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