Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces

Norifumi Kawakami, Hiroki Kondo, Yuki Matsuzawa, Kaoru Hayasaka, Erika Nasu, Kenji Sasahara, Ryoichi Arai, Kenji Miyamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Protein-based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano-building block for further molecular assembly.

Original languageEnglish
Pages (from-to)12400-12404
Number of pages5
JournalAngewandte Chemie - International Edition
Volume57
Issue number38
DOIs
Publication statusPublished - 2018 Sep 17

Keywords

  • cage proteins
  • fusion protein
  • nanoparticles
  • protein design
  • self-assembly

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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