@article{5865b637e3804ecfb2ff272ba09c9075,
title = "Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces",
abstract = "Protein-based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano-building block for further molecular assembly.",
keywords = "cage proteins, fusion protein, nanoparticles, protein design, self-assembly",
author = "Norifumi Kawakami and Hiroki Kondo and Yuki Matsuzawa and Kaoru Hayasaka and Erika Nasu and Kenji Sasahara and Ryoichi Arai and Kenji Miyamoto",
note = "Funding Information: We acknowledge the Division for Medical Research Engineering, Nagoya University Graduate School of Medicine, for technical support of TEM observation. We thank Prof. Y. Watanabe and Dr. O. Shoji of Nagoya University for DLS measurements, Dr. T. Kosugi and Dr. N. Koga of Institute for Molecular Science (IMS) for SEC-MALS and CD experiments, and Dr. N. Shimizu, Dr. N. Igarashi, and Photon Factory (PF) staff for their help in synchrotron SAXS experiments, which were performed at PF, KEK, under the approval of PF program advisory committee (proposal numbers 2016G153 and 2016G606). This work was supported by Mukai Science and Technology foundation, Keio Gijuku Academic Development Funds and JSPS KAKENHI Grant numbers JP18K05324 to N.K., and the Joint Studies Program (2017?2018) of IMS and JSPS KAKENHI Grant numbers JP16H00761 and JP16K05841 to R.A. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2018",
month = sep,
day = "17",
doi = "10.1002/anie.201805565",
language = "English",
volume = "57",
pages = "12400--12404",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "38",
}