Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture

Aya Mizutani Akimoto, Erika Hasuike, Hiroto Tada, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.

Original languageEnglish
Pages (from-to)1203-1206
Number of pages4
JournalAnalytical Sciences
Volume32
Issue number11
Publication statusPublished - 2016

Fingerprint

Hydrogel
Cell culture
Polyethylene glycols
Gels
Gelation
Polymers
arginyl-glycyl-aspartyl-serine
Adhesives
Adhesion
Mechanical properties
Temperature

Keywords

  • 3D cell culture
  • Hydrogel
  • Poly(N-isopropylacrylamide)
  • Thermoresponsive gel

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Akimoto, A. M., Hasuike, E., Tada, H., Nagase, K., Okano, T., Kanazawa, H., & Yoshida, R. (2016). Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture. Analytical Sciences, 32(11), 1203-1206.

Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture. / Akimoto, Aya Mizutani; Hasuike, Erika; Tada, Hiroto; Nagase, Kenichi; Okano, Teruo; Kanazawa, Hideko; Yoshida, Ryo.

In: Analytical Sciences, Vol. 32, No. 11, 2016, p. 1203-1206.

Research output: Contribution to journalArticle

Akimoto, AM, Hasuike, E, Tada, H, Nagase, K, Okano, T, Kanazawa, H & Yoshida, R 2016, 'Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture', Analytical Sciences, vol. 32, no. 11, pp. 1203-1206.
Akimoto, Aya Mizutani ; Hasuike, Erika ; Tada, Hiroto ; Nagase, Kenichi ; Okano, Teruo ; Kanazawa, Hideko ; Yoshida, Ryo. / Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture. In: Analytical Sciences. 2016 ; Vol. 32, No. 11. pp. 1203-1206.
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AU - Kanazawa, Hideko

AU - Yoshida, Ryo

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