Abstract
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.
| Original language | English |
|---|---|
| Pages (from-to) | 1203-1206 |
| Number of pages | 4 |
| Journal | Analytical Sciences |
| Volume | 32 |
| Issue number | 11 |
| Publication status | Published - 2016 |
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Keywords
- 3D cell culture
- Hydrogel
- Poly(N-isopropylacrylamide)
- Thermoresponsive gel
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture. / Akimoto, Aya Mizutani; Hasuike, Erika; Tada, Hiroto; Nagase, Kenichi; Okano, Teruo; Kanazawa, Hideko; Yoshida, Ryo.
In: Analytical Sciences, Vol. 32, No. 11, 2016, p. 1203-1206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture
AU - Akimoto, Aya Mizutani
AU - Hasuike, Erika
AU - Tada, Hiroto
AU - Nagase, Kenichi
AU - Okano, Teruo
AU - Kanazawa, Hideko
AU - Yoshida, Ryo
PY - 2016
Y1 - 2016
N2 - We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.
AB - We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.
KW - 3D cell culture
KW - Hydrogel
KW - Poly(N-isopropylacrylamide)
KW - Thermoresponsive gel
UR - http://www.scopus.com/inward/record.url?scp=84995897347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84995897347&partnerID=8YFLogxK
M3 - Article
C2 - 27829626
AN - SCOPUS:84995897347
VL - 32
SP - 1203
EP - 1206
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 11
ER -