Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture

Aya Mizutani Akimoto; Erika Hasuike; Hiroto Tada; Kenichi Nagase; Teruo Okano; Hideko Kanazawa; Ryo Yoshida

doi:10.2116/analsci.32.1203

Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture

Aya Mizutani Akimoto, Erika Hasuike, Hiroto Tada, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.

Original language	English
Pages (from-to)	1203-1206
Number of pages	4
Journal	analytical sciences
Volume	32
Issue number	11
DOIs	https://doi.org/10.2116/analsci.32.1203
Publication status	Published - 2016 Jan 1

Keywords

3D cell culture
Hydrogel
Poly(N-isopropylacrylamide)
Thermoresponsive gel

ASJC Scopus subject areas

Analytical Chemistry

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Akimoto, A. M., Hasuike, E., Tada, H., Nagase, K., Okano, T., Kanazawa, H., & Yoshida, R. (2016). Design of tetra-arm PEG-crosslinked thermoresponsive hydrogel for 3D cell culture. analytical sciences, 32(11), 1203-1206. https://doi.org/10.2116/analsci.32.1203

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abstract = "We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.",

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AU - Okano, Teruo

AU - Kanazawa, Hideko

AU - Yoshida, Ryo

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