Abstract
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.
Original language | English |
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Pages (from-to) | 1203-1206 |
Number of pages | 4 |
Journal | analytical sciences |
Volume | 32 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- 3D cell culture
- Hydrogel
- Poly(N-isopropylacrylamide)
- Thermoresponsive gel
ASJC Scopus subject areas
- Analytical Chemistry