Designing a multistage, SNP-based, genome screen for common diseases

Yasunori Sato, Hideki Suganami, Chikuma Hamada, Isao Yoshimura, Teruhiko Yoshida, Kimio Yoshimura

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A genome-wide linkage equilibrium mapping is an emerging strategy to identify risk-modifying genes for common diseases, despite unsettled controversies upon many aspects, including its premises, designs, marker choices and cost benefits. One large-scale attempt in Japan aims to identify disease-associated single nucleotide polymorphisms (SNPs) for five diseases among the Japanese population: Alzheimer's disease, gastric cancer, diabetes, hypertension and asthma. Following an initial screening of c.a. 100,000 SNPs on 940 subjects (five diseases x 188 patients) to select about 2,000 SNPs, we compared which subsequent screening design is more appropriate, and an additional one or two screens to further narrow down any disease-associated SNPs within a fixed total volume of 15,040,000 typings (2,000 SNPs x five diseases x 1,504 subjects, comprising 752 cases and 752 controls). We employed a Monte Carlo simulation to evaluate the probability of identifying truly disease-associated SNPs. The results suggest the single additional stage design (i.e., total two-stage design including the initial screening of 100,000 SNPs) was more practicable for the simple reason that the gain in probability is considered insufficient relative to an associated increase in study complexity in the three-stage design.

Original languageEnglish
Pages (from-to)669-676
Number of pages8
JournalJournal of Human Genetics
Volume49
Issue number12
DOIs
Publication statusPublished - 2004 Dec
Externally publishedYes

Keywords

  • Case-control study
  • Disease association study
  • Genome-wide approaches
  • Multistage design
  • Odds ratio
  • Sensitivity
  • Single nucleotide polymorphisms (SNPs)

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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