Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK

Takashi Hashimoto, Masayuki Amagai, David A D Parry, Tony W. Dixon, Sachiko Tsukita, Shoichiro Tsukita, Keizaburo Miki, Kosuke Sakai, Yoshio Inokuchi, Jun Kudo, Nobuyoshi Shimizu, Takeji Nishikawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have obtained a monoclonal antibody (33A-3D) that specifically recognize desmoyokin, a 680 KDa desmosomal plaque protein that is well characterized in bovine muzzle epidermis. A cDNA clone (DY6, 3693 bp) was isolated by immunoscreening a mouse keratinocyte expression library with 33A-3D, and it was confirmed that DY6 has a partial coding sequence for desmoyokin. DY6 consists of highly homologous repeats about 128 residues long. Furthermore, the 128-residue repeats exhibit a quasi seven-residue substructure, which we believe will adopt an antiparallel β-sheet structure. Surprisingly, the amino acid sequence showed a significant homology with AHNAK, a newly identified human gene encoding a 700 kDa protein, which was suggested to be down-regulated in neuroblastoma. From its extensive homology, the similarity in both size and structure, and the identical patterns on Southern blot analysis of genomic DNAs, desmoyokin and AHNAK protein are thought to be identical. Although the desmoyokin/AHNAK protein is detected in a variety of cell types at both protein and mRNA levels, its distribution in keratinocytes (associated closely with cell membrane) is quite different from that in cells other than keratinocytes (distributed diffusely in the cytoplasm). These findings suggest that the desmoyokin/AHNAK protein is a ubiquitous molecule with a unique structure and appears to have different distributions (and probably different functions) among different cells.

Original languageEnglish
Pages (from-to)275-286
Number of pages12
JournalJournal of Cell Science
Volume105
Issue number2
Publication statusPublished - 1993 Jun

Fingerprint

Keratinocytes
Blood Proteins
Membrane Proteins
Cell Membrane
Genes
Proteins
Southern Blotting
Neuroblastoma
Epidermis
Amino Acid Sequence
Cytoplasm
Complementary DNA
Clone Cells
Monoclonal Antibodies
Messenger RNA
DNA

Keywords

  • AHNAK
  • Desmosomes
  • Desmoyokin
  • Keratinocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hashimoto, T., Amagai, M., Parry, D. A. D., Dixon, T. W., Tsukita, S., Tsukita, S., ... Nishikawa, T. (1993). Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK. Journal of Cell Science, 105(2), 275-286.

Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK. / Hashimoto, Takashi; Amagai, Masayuki; Parry, David A D; Dixon, Tony W.; Tsukita, Sachiko; Tsukita, Shoichiro; Miki, Keizaburo; Sakai, Kosuke; Inokuchi, Yoshio; Kudo, Jun; Shimizu, Nobuyoshi; Nishikawa, Takeji.

In: Journal of Cell Science, Vol. 105, No. 2, 06.1993, p. 275-286.

Research output: Contribution to journalArticle

Hashimoto, T, Amagai, M, Parry, DAD, Dixon, TW, Tsukita, S, Tsukita, S, Miki, K, Sakai, K, Inokuchi, Y, Kudo, J, Shimizu, N & Nishikawa, T 1993, 'Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK', Journal of Cell Science, vol. 105, no. 2, pp. 275-286.
Hashimoto, Takashi ; Amagai, Masayuki ; Parry, David A D ; Dixon, Tony W. ; Tsukita, Sachiko ; Tsukita, Shoichiro ; Miki, Keizaburo ; Sakai, Kosuke ; Inokuchi, Yoshio ; Kudo, Jun ; Shimizu, Nobuyoshi ; Nishikawa, Takeji. / Desmoyokin, a 680 kDa keratinocyte plasma membrane-associated protein, is homologous to the protein encoded by human gene AHNAK. In: Journal of Cell Science. 1993 ; Vol. 105, No. 2. pp. 275-286.
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abstract = "We have obtained a monoclonal antibody (33A-3D) that specifically recognize desmoyokin, a 680 KDa desmosomal plaque protein that is well characterized in bovine muzzle epidermis. A cDNA clone (DY6, 3693 bp) was isolated by immunoscreening a mouse keratinocyte expression library with 33A-3D, and it was confirmed that DY6 has a partial coding sequence for desmoyokin. DY6 consists of highly homologous repeats about 128 residues long. Furthermore, the 128-residue repeats exhibit a quasi seven-residue substructure, which we believe will adopt an antiparallel β-sheet structure. Surprisingly, the amino acid sequence showed a significant homology with AHNAK, a newly identified human gene encoding a 700 kDa protein, which was suggested to be down-regulated in neuroblastoma. From its extensive homology, the similarity in both size and structure, and the identical patterns on Southern blot analysis of genomic DNAs, desmoyokin and AHNAK protein are thought to be identical. Although the desmoyokin/AHNAK protein is detected in a variety of cell types at both protein and mRNA levels, its distribution in keratinocytes (associated closely with cell membrane) is quite different from that in cells other than keratinocytes (distributed diffusely in the cytoplasm). These findings suggest that the desmoyokin/AHNAK protein is a ubiquitous molecule with a unique structure and appears to have different distributions (and probably different functions) among different cells.",
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