Detachable immobilization of liposomes on polymer gel particles

Md Abdul Khaleque, Yukihisa Okumura, Satoshi Yabushita, Michiharu Mitani

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Immobilization of liposomes on hydrophobized Sephacryl gel and controlled detachment of the liposomes from the gel were examined. The gel was chemically modified and bore octyl, hexadecyl or cholesteryl moiety via disulfide linkage as anchors to liposomal bilayer membrane. Upon interaction with the gel, egg phosphatidylcholine liposomes were successfully immobilized onto the gel. The gel with cholesteryl moiety showed 1.7 times higher liposome immobilization per anchor moiety than the gels with the alkyl moieties. The immobilization of liposomes on the gel was stable, and no significant spontaneous detachment of phospholipid or leakage of fluorescein isothiocyanate-conjugated dextran encapsulated in the immobilized liposomes was observed in 24 h. Reductive cleavage of the disulfide linkage by dithiothreitol resulted in detachment of the liposomes from the gel. The majority of the detached liposomes were found encapsulating the dextran derivative, and these liposomes should have kept their structural integrity throughout the immobilization and the detachment processes. The release of the liposomes was insignificant until the ratio of the dithiothreitol to the hydrophobic anchor reached a threshold. The presence of the threshold suggests that the immobilization of liposomes should require a certain minimum number of the hydrophobic moieties anchored in the liposomal membrane. By applying the present immobilization-detachment system, preparation of liposomes encapsulating the dextran derivative without using costly gel filtration or ultracentrifugation procedure was successfully demonstrated.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume37
Issue number1-2
DOIs
Publication statusPublished - 2004 Aug 15
Externally publishedYes

Fingerprint

Liposomes
immobilization
Immobilization
Particles (particulate matter)
Polymers
Gels
gels
polymers
detachment
dextrans
Dextran
Dextrans
Anchors
encapsulating
disulfides
linkages
Dithiothreitol
Disulfides
membranes
thresholds

Keywords

  • Cross-linked polymer gel
  • Disulfide linkage
  • Hydrophobic anchor
  • Liposome immobilization and detachment
  • Liposome separation

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Detachable immobilization of liposomes on polymer gel particles. / Khaleque, Md Abdul; Okumura, Yukihisa; Yabushita, Satoshi; Mitani, Michiharu.

In: Colloids and Surfaces B: Biointerfaces, Vol. 37, No. 1-2, 15.08.2004, p. 35-42.

Research output: Contribution to journalArticle

Khaleque, Md Abdul ; Okumura, Yukihisa ; Yabushita, Satoshi ; Mitani, Michiharu. / Detachable immobilization of liposomes on polymer gel particles. In: Colloids and Surfaces B: Biointerfaces. 2004 ; Vol. 37, No. 1-2. pp. 35-42.
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