Detecting and tracking multiple fluorescence spot in noisy DXT image sequence

Reo Sakakura, Yoshimitsu Aoki, Keishi Shimizu, Shigetoshi Oiki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Quantitative analysis of molecular structural change by means of Diffracted X-ray tracking (DXT) method requires tracking of dozens of bright spots in noisy image sequence. As the observation equipment upgrades, experimental data was cannot be analyzed manually with sufficient speed and accuracy. We propose automatic analysis method combining background estimation and frame-by-frame matching. We applied propose method to real DXT image data acquired for potassium ion channel structural changing analysis for evaluation. The result of experiments show that the method has high accuracy and be able to replace manual analysis.

Original languageEnglish
Title of host publicationProceedings of the SICE Annual Conference
Pages96-98
Number of pages3
Publication statusPublished - 2010
EventSICE Annual Conference 2010, SICE 2010 - Taipei, Taiwan, Province of China
Duration: 2010 Aug 182010 Aug 21

Other

OtherSICE Annual Conference 2010, SICE 2010
CountryTaiwan, Province of China
CityTaipei
Period10/8/1810/8/21

Fingerprint

Fluorescence
X rays
Potassium
Ions
Chemical analysis
Experiments

Keywords

  • Background etimation
  • Component
  • Fluorescence image sequence
  • Kernel density estimation
  • Multiple object tracking
  • Occurrence probability

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Control and Systems Engineering
  • Computer Science Applications

Cite this

Sakakura, R., Aoki, Y., Shimizu, K., & Oiki, S. (2010). Detecting and tracking multiple fluorescence spot in noisy DXT image sequence. In Proceedings of the SICE Annual Conference (pp. 96-98). [5602418]

Detecting and tracking multiple fluorescence spot in noisy DXT image sequence. / Sakakura, Reo; Aoki, Yoshimitsu; Shimizu, Keishi; Oiki, Shigetoshi.

Proceedings of the SICE Annual Conference. 2010. p. 96-98 5602418.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sakakura, R, Aoki, Y, Shimizu, K & Oiki, S 2010, Detecting and tracking multiple fluorescence spot in noisy DXT image sequence. in Proceedings of the SICE Annual Conference., 5602418, pp. 96-98, SICE Annual Conference 2010, SICE 2010, Taipei, Taiwan, Province of China, 10/8/18.
Sakakura R, Aoki Y, Shimizu K, Oiki S. Detecting and tracking multiple fluorescence spot in noisy DXT image sequence. In Proceedings of the SICE Annual Conference. 2010. p. 96-98. 5602418
Sakakura, Reo ; Aoki, Yoshimitsu ; Shimizu, Keishi ; Oiki, Shigetoshi. / Detecting and tracking multiple fluorescence spot in noisy DXT image sequence. Proceedings of the SICE Annual Conference. 2010. pp. 96-98
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