Detection and characterization of endothelin-1-like immunoreactivity in rat plasma

Yoshihiko Saito, Kazuwa Nakao, Gotaro Shirakami, Michihisa Jougasaki, Takayuki Yamada, Hiroshi Itoh, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Shin ichi Suga, Yoshihiro Ogawa, Hiroo Imura

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Abstract

Using two radioimmunoassays (RIAs) for endothelin-1 (ET-1) with and without a substantial cross-reactivity with ET-3, we have measured the plasma ET-1-like immunoreactivity (-LI) level in rat plasma. ET-1-LI was detected in plasma from male Wistar rats. ET-1-LI in rat plasma consisted of three components with molecular weights of 6K, 4K and 2.5K daltons by gel permeation chromatography. Two of the components were eluted at positions of big ET (4K) and synthetic ET-1 (2.5K). The remaining component was eluted at the preceding fraction (6K). No difference was observed in ET-1-LI of the small molecular form of ET (2.5K) between the two RIAs. Thus, there is little or no ET-3 in rat plasma, which has the sequence found originally in the rat genome. The concentration of the small molecular form of ET, presumably ET-1, in rat plasma was about 4 pg/ml.

Original languageEnglish
Pages (from-to)1512-1516
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume163
Issue number3
DOIs
Publication statusPublished - 1989 Sep 29
Externally publishedYes

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Endothelin-1
Rats
Plasmas
Radioimmunoassay
Gel permeation chromatography
Gel Chromatography
Wistar Rats
Genes
Molecular Weight
Molecular weight
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection and characterization of endothelin-1-like immunoreactivity in rat plasma. / Saito, Yoshihiko; Nakao, Kazuwa; Shirakami, Gotaro; Jougasaki, Michihisa; Yamada, Takayuki; Itoh, Hiroshi; Mukoyama, Masashi; Arai, Hiroshi; Hosoda, Kiminori; Suga, Shin ichi; Ogawa, Yoshihiro; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 163, No. 3, 29.09.1989, p. 1512-1516.

Research output: Contribution to journalArticle

Saito, Y, Nakao, K, Shirakami, G, Jougasaki, M, Yamada, T, Itoh, H, Mukoyama, M, Arai, H, Hosoda, K, Suga, SI, Ogawa, Y & Imura, H 1989, 'Detection and characterization of endothelin-1-like immunoreactivity in rat plasma', Biochemical and Biophysical Research Communications, vol. 163, no. 3, pp. 1512-1516. https://doi.org/10.1016/0006-291X(89)91151-0
Saito, Yoshihiko ; Nakao, Kazuwa ; Shirakami, Gotaro ; Jougasaki, Michihisa ; Yamada, Takayuki ; Itoh, Hiroshi ; Mukoyama, Masashi ; Arai, Hiroshi ; Hosoda, Kiminori ; Suga, Shin ichi ; Ogawa, Yoshihiro ; Imura, Hiroo. / Detection and characterization of endothelin-1-like immunoreactivity in rat plasma. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 163, No. 3. pp. 1512-1516.
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