Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging

Eiji Sugiyama, Matteo M. Guerrini, Kurara Honda, Yuko Hattori, Manabu Abe, Patrik Källback, Per E. Andrén, Kenji F. Tanaka, Mitsutoshi Setou, Sidonia Fagarasan, Makoto Suematsu, Yuki Sugiura

Research output: Contribution to journalArticle

Abstract

Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system.

Original languageEnglish
Pages (from-to)359-372
Number of pages14
JournaliScience
Volume20
DOIs
Publication statusPublished - 2019 Oct 25

Fingerprint

Mass Spectrometry
Serotonin
Midline Thalamic Nuclei
Brain
Norepinephrine
Tryptophan
Dopamine
Neurons
Raphe Nuclei
Atlases
Amygdala
Isotopes
Cognition
Catecholamines
Neurotransmitter Agents

Keywords

  • Neuroscience
  • Spectroscopy
  • Systems Neuroscience

ASJC Scopus subject areas

  • General

Cite this

Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging. / Sugiyama, Eiji; Guerrini, Matteo M.; Honda, Kurara; Hattori, Yuko; Abe, Manabu; Källback, Patrik; Andrén, Per E.; Tanaka, Kenji F.; Setou, Mitsutoshi; Fagarasan, Sidonia; Suematsu, Makoto; Sugiura, Yuki.

In: iScience, Vol. 20, 25.10.2019, p. 359-372.

Research output: Contribution to journalArticle

Sugiyama, E, Guerrini, MM, Honda, K, Hattori, Y, Abe, M, Källback, P, Andrén, PE, Tanaka, KF, Setou, M, Fagarasan, S, Suematsu, M & Sugiura, Y 2019, 'Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging', iScience, vol. 20, pp. 359-372. https://doi.org/10.1016/j.isci.2019.09.036
Sugiyama E, Guerrini MM, Honda K, Hattori Y, Abe M, Källback P et al. Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging. iScience. 2019 Oct 25;20:359-372. https://doi.org/10.1016/j.isci.2019.09.036
Sugiyama, Eiji ; Guerrini, Matteo M. ; Honda, Kurara ; Hattori, Yuko ; Abe, Manabu ; Källback, Patrik ; Andrén, Per E. ; Tanaka, Kenji F. ; Setou, Mitsutoshi ; Fagarasan, Sidonia ; Suematsu, Makoto ; Sugiura, Yuki. / Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging. In: iScience. 2019 ; Vol. 20. pp. 359-372.
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