Abstract
A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.
| Original language | English |
|---|---|
| Title of host publication | Transactions - 7th World Biomaterials Congress |
| Pages | 1381 |
| Number of pages | 1 |
| Publication status | Published - 2004 |
| Event | Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: 2004 May 17 → 2004 May 21 |
Other
| Other | Transactions - 7th World Biomaterials Congress |
|---|---|
| Country | Australia |
| City | Sydney |
| Period | 04/5/17 → 04/5/21 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. / Sato, Y.; Okumura, A.; Suzuki, K.; Kawaguchi, H.
Transactions - 7th World Biomaterials Congress. 2004. p. 1381.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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TY - GEN
T1 - Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance
AU - Sato, Y.
AU - Okumura, A.
AU - Suzuki, K.
AU - Kawaguchi, H.
PY - 2004
Y1 - 2004
N2 - A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.
AB - A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.
UR - http://www.scopus.com/inward/record.url?scp=13844267614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844267614&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:13844267614
SN - 1877040193
SN - 9781877040191
SP - 1381
BT - Transactions - 7th World Biomaterials Congress
ER -