Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance

Y. Sato, A. Okumura, K. Suzuki, H. Kawaguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1381
Number of pages1
Publication statusPublished - 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

Fingerprint

Surface plasmon resonance
Microspheres
DNA
Biosensors
Genes
Ligands
Flow rate
Geometry
Peptide Nucleic Acids

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sato, Y., Okumura, A., Suzuki, K., & Kawaguchi, H. (2004). Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. In Transactions - 7th World Biomaterials Congress (pp. 1381)

Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. / Sato, Y.; Okumura, A.; Suzuki, K.; Kawaguchi, H.

Transactions - 7th World Biomaterials Congress. 2004. p. 1381.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sato, Y, Okumura, A, Suzuki, K & Kawaguchi, H 2004, Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. in Transactions - 7th World Biomaterials Congress. pp. 1381, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 04/5/17.
Sato Y, Okumura A, Suzuki K, Kawaguchi H. Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. In Transactions - 7th World Biomaterials Congress. 2004. p. 1381
Sato, Y. ; Okumura, A. ; Suzuki, K. ; Kawaguchi, H. / Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance. Transactions - 7th World Biomaterials Congress. 2004. pp. 1381
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