Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance

Y. Sato; A. Okumura; K. Suzuki; H. Kawaguchi

Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance

Y. Sato, A. Okumura, K. Suzuki, H. Kawaguchi

Department of Applied Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 10² and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.

Original language	English
Title of host publication	Transactions - 7th World Biomaterials Congress
Number of pages	1
Publication status	Published - 2004 Dec 1
Event	Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: 2004 May 17 → 2004 May 21

Publication series

Name	Transactions - 7th World Biomaterials Congress

Other

Other	Transactions - 7th World Biomaterials Congress
Country	Australia
City	Sydney
Period	04/5/17 → 04/5/21

ASJC Scopus subject areas

Engineering(all)

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Cite this

@inproceedings{1426c96ed33d4617a6e8f7d4f12f7865,

title = "Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance",

abstract = "A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.",

author = "Y. Sato and A. Okumura and K. Suzuki and H. Kawaguchi",

year = "2004",

month = dec,

day = "1",

language = "English",

isbn = "1877040193",

series = "Transactions - 7th World Biomaterials Congress",

booktitle = "Transactions - 7th World Biomaterials Congress",

note = "Transactions - 7th World Biomaterials Congress ; Conference date: 17-05-2004 Through 21-05-2004",

}

TY - GEN

T1 - Detection of a K-ras point mutation with DNA-conjugated microspheres by surface plasmon resonance

AU - Sato, Y.

AU - Okumura, A.

AU - Suzuki, K.

AU - Kawaguchi, H.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.

AB - A method for increasing the specificity of detecting a DNA point mutation and the sensitivity of evanescent wave based biosensors was presented. A peptide nucleic acid (PNA) ligand was used to increase the specificity to detect G-G mismatch in K-ras gene affiliated with pancreatic cancer. It was observed that affinity constants between complementary and mismatched targets was of the order of 102 and the largest for PNA interacting with 11mer targets. The results show that change in flow rate has a different effect on the stability of microspheres in a sandwitched geometry that are hybridized to the surface.

UR - http://www.scopus.com/inward/record.url?scp=13844267614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844267614&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:13844267614

SN - 1877040193

SN - 9781877040191

T3 - Transactions - 7th World Biomaterials Congress

BT - Transactions - 7th World Biomaterials Congress

T2 - Transactions - 7th World Biomaterials Congress

Y2 - 17 May 2004 through 21 May 2004

ER -