Detection of a novel herpesvirus from bats in the Philippines

Kaori Sano, Sachiko Okazaki, Satoshi Taniguchi, Joseph S. Masangkay, Roberto Puentespina, Eduardo Eres, Edison Cosico, Niña Quibod, Taisuke Kondo, Hiroshi Shimoda, Yuuki Hatta, Shumpei Mitomo, Mami Oba, Yukie Katayama, Yukiko Sassa, Tetsuya Furuya, Makoto Nagai, Yumi Une, Ken Maeda, Shigeru KyuwaYasuhiro Yoshikawa, Hiroomi Akashi, Tsutomu Omatsu, Tetsuya Mizutani

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region.

Original languageEnglish
Pages (from-to)136-139
Number of pages4
JournalVirus Genes
Volume51
Issue number1
DOIs
Publication statusPublished - 2015 Aug 29
Externally publishedYes

Fingerprint

Philippines
Herpesviridae
Polymerase Chain Reaction
Viruses
Gammaherpesvirinae
Viral Genes
Zoonoses
Retroviridae
Intestines
Communicable Diseases
Thrombosis
Genome
Lung
Infection
Population

Keywords

  • Bats
  • Herpesvirus
  • Next generation sequencing
  • The Philippines

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Sano, K., Okazaki, S., Taniguchi, S., Masangkay, J. S., Puentespina, R., Eres, E., ... Mizutani, T. (2015). Detection of a novel herpesvirus from bats in the Philippines. Virus Genes, 51(1), 136-139. https://doi.org/10.1007/s11262-015-1197-6

Detection of a novel herpesvirus from bats in the Philippines. / Sano, Kaori; Okazaki, Sachiko; Taniguchi, Satoshi; Masangkay, Joseph S.; Puentespina, Roberto; Eres, Eduardo; Cosico, Edison; Quibod, Niña; Kondo, Taisuke; Shimoda, Hiroshi; Hatta, Yuuki; Mitomo, Shumpei; Oba, Mami; Katayama, Yukie; Sassa, Yukiko; Furuya, Tetsuya; Nagai, Makoto; Une, Yumi; Maeda, Ken; Kyuwa, Shigeru; Yoshikawa, Yasuhiro; Akashi, Hiroomi; Omatsu, Tsutomu; Mizutani, Tetsuya.

In: Virus Genes, Vol. 51, No. 1, 29.08.2015, p. 136-139.

Research output: Contribution to journalArticle

Sano, K, Okazaki, S, Taniguchi, S, Masangkay, JS, Puentespina, R, Eres, E, Cosico, E, Quibod, N, Kondo, T, Shimoda, H, Hatta, Y, Mitomo, S, Oba, M, Katayama, Y, Sassa, Y, Furuya, T, Nagai, M, Une, Y, Maeda, K, Kyuwa, S, Yoshikawa, Y, Akashi, H, Omatsu, T & Mizutani, T 2015, 'Detection of a novel herpesvirus from bats in the Philippines', Virus Genes, vol. 51, no. 1, pp. 136-139. https://doi.org/10.1007/s11262-015-1197-6
Sano K, Okazaki S, Taniguchi S, Masangkay JS, Puentespina R, Eres E et al. Detection of a novel herpesvirus from bats in the Philippines. Virus Genes. 2015 Aug 29;51(1):136-139. https://doi.org/10.1007/s11262-015-1197-6
Sano, Kaori ; Okazaki, Sachiko ; Taniguchi, Satoshi ; Masangkay, Joseph S. ; Puentespina, Roberto ; Eres, Eduardo ; Cosico, Edison ; Quibod, Niña ; Kondo, Taisuke ; Shimoda, Hiroshi ; Hatta, Yuuki ; Mitomo, Shumpei ; Oba, Mami ; Katayama, Yukie ; Sassa, Yukiko ; Furuya, Tetsuya ; Nagai, Makoto ; Une, Yumi ; Maeda, Ken ; Kyuwa, Shigeru ; Yoshikawa, Yasuhiro ; Akashi, Hiroomi ; Omatsu, Tsutomu ; Mizutani, Tetsuya. / Detection of a novel herpesvirus from bats in the Philippines. In: Virus Genes. 2015 ; Vol. 51, No. 1. pp. 136-139.
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