Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases

Kiyomitsu Miyachi, Hiroshi Matsushima, Yuriko Nakagawa, Kazuto Murata, Raleigh W. Hawkins, Michito Hirakata, Akira Suwa, Masanori Adachi, Kuniomi Yamasaki, Marvin J. Fritzler

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An early endosome antigen previously reported by F.T. Mu could be stained on cytoplasmic vesicles of HEp-2 cells. Here, we have investigated autoantibodies against cytoplasmic vesicular antigens, especially against early endosome antigen 1. Twelve sera were selected on the basis of cytoplasmic vesicular staining patterns of HEp-2 cells. Protein-immunoprecipitation using 35S-methionine labeled HeLa lysates, and RNA immunoprecipitation using 32P-labeled HeLa lysates were conducted to characterize the cognate antigens. Nine of 12 sera reacted with proteins in the range of 162-180 kDa, three of which were found to react specifically with the 162 kDa 35S methionine labeled recombinant early endosome antigen 1. These proteins were not associated with common RNA. Although complete clinical information was not available, some of the patients had rheumatoid arthritis(RA). In addition, the RNA-IPP results suggest that other patients included one each with SLE, SSc, polymyositis, and Sjögren's syndrome. Anti-early endosome antigen 1 antibody was found in 25%(3 of 12) of sera known to stain cytoplasmic vesicles. The reactive sera came mostly from patients with RA. The sera was from one case each of clinical-confirmed RA, SLE and Sjögren's syndrome.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalRinsho byori. The Japanese journal of clinical pathology
Volume51
Issue number6
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Connective Tissue Diseases
Staining and Labeling
Antibodies
Cytoplasmic Vesicles
Rheumatoid Arthritis
Serum
RNA
Immunoprecipitation
Antigens
Methionine
Polymyositis
Proteins
Endosomes
Autoantibodies
Coloring Agents
early endosome antigen 1

Cite this

Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases. / Miyachi, Kiyomitsu; Matsushima, Hiroshi; Nakagawa, Yuriko; Murata, Kazuto; Hawkins, Raleigh W.; Hirakata, Michito; Suwa, Akira; Adachi, Masanori; Yamasaki, Kuniomi; Fritzler, Marvin J.

In: Rinsho byori. The Japanese journal of clinical pathology, Vol. 51, No. 6, 2003, p. 501-507.

Research output: Contribution to journalArticle

Miyachi, K, Matsushima, H, Nakagawa, Y, Murata, K, Hawkins, RW, Hirakata, M, Suwa, A, Adachi, M, Yamasaki, K & Fritzler, MJ 2003, 'Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases', Rinsho byori. The Japanese journal of clinical pathology, vol. 51, no. 6, pp. 501-507.
Miyachi, Kiyomitsu ; Matsushima, Hiroshi ; Nakagawa, Yuriko ; Murata, Kazuto ; Hawkins, Raleigh W. ; Hirakata, Michito ; Suwa, Akira ; Adachi, Masanori ; Yamasaki, Kuniomi ; Fritzler, Marvin J. / Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases. In: Rinsho byori. The Japanese journal of clinical pathology. 2003 ; Vol. 51, No. 6. pp. 501-507.
@article{a015dab949d241408285eb5520d053a1,
title = "Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases",
abstract = "An early endosome antigen previously reported by F.T. Mu could be stained on cytoplasmic vesicles of HEp-2 cells. Here, we have investigated autoantibodies against cytoplasmic vesicular antigens, especially against early endosome antigen 1. Twelve sera were selected on the basis of cytoplasmic vesicular staining patterns of HEp-2 cells. Protein-immunoprecipitation using 35S-methionine labeled HeLa lysates, and RNA immunoprecipitation using 32P-labeled HeLa lysates were conducted to characterize the cognate antigens. Nine of 12 sera reacted with proteins in the range of 162-180 kDa, three of which were found to react specifically with the 162 kDa 35S methionine labeled recombinant early endosome antigen 1. These proteins were not associated with common RNA. Although complete clinical information was not available, some of the patients had rheumatoid arthritis(RA). In addition, the RNA-IPP results suggest that other patients included one each with SLE, SSc, polymyositis, and Sj{\"o}gren's syndrome. Anti-early endosome antigen 1 antibody was found in 25{\%}(3 of 12) of sera known to stain cytoplasmic vesicles. The reactive sera came mostly from patients with RA. The sera was from one case each of clinical-confirmed RA, SLE and Sj{\"o}gren's syndrome.",
author = "Kiyomitsu Miyachi and Hiroshi Matsushima and Yuriko Nakagawa and Kazuto Murata and Hawkins, {Raleigh W.} and Michito Hirakata and Akira Suwa and Masanori Adachi and Kuniomi Yamasaki and Fritzler, {Marvin J.}",
year = "2003",
language = "English",
volume = "51",
pages = "501--507",
journal = "Rinsho byori. The Japanese journal of clinical pathology",
issn = "0047-1860",
publisher = "Nihon Rinsho Byori Gakkai",
number = "6",

}

TY - JOUR

T1 - Detection of anti-early endosome antigen 1 antibody in sera showing cytoplasmic vesicular staining, taken from patients with connective tissue diseases

AU - Miyachi, Kiyomitsu

AU - Matsushima, Hiroshi

AU - Nakagawa, Yuriko

AU - Murata, Kazuto

AU - Hawkins, Raleigh W.

AU - Hirakata, Michito

AU - Suwa, Akira

AU - Adachi, Masanori

AU - Yamasaki, Kuniomi

AU - Fritzler, Marvin J.

PY - 2003

Y1 - 2003

N2 - An early endosome antigen previously reported by F.T. Mu could be stained on cytoplasmic vesicles of HEp-2 cells. Here, we have investigated autoantibodies against cytoplasmic vesicular antigens, especially against early endosome antigen 1. Twelve sera were selected on the basis of cytoplasmic vesicular staining patterns of HEp-2 cells. Protein-immunoprecipitation using 35S-methionine labeled HeLa lysates, and RNA immunoprecipitation using 32P-labeled HeLa lysates were conducted to characterize the cognate antigens. Nine of 12 sera reacted with proteins in the range of 162-180 kDa, three of which were found to react specifically with the 162 kDa 35S methionine labeled recombinant early endosome antigen 1. These proteins were not associated with common RNA. Although complete clinical information was not available, some of the patients had rheumatoid arthritis(RA). In addition, the RNA-IPP results suggest that other patients included one each with SLE, SSc, polymyositis, and Sjögren's syndrome. Anti-early endosome antigen 1 antibody was found in 25%(3 of 12) of sera known to stain cytoplasmic vesicles. The reactive sera came mostly from patients with RA. The sera was from one case each of clinical-confirmed RA, SLE and Sjögren's syndrome.

AB - An early endosome antigen previously reported by F.T. Mu could be stained on cytoplasmic vesicles of HEp-2 cells. Here, we have investigated autoantibodies against cytoplasmic vesicular antigens, especially against early endosome antigen 1. Twelve sera were selected on the basis of cytoplasmic vesicular staining patterns of HEp-2 cells. Protein-immunoprecipitation using 35S-methionine labeled HeLa lysates, and RNA immunoprecipitation using 32P-labeled HeLa lysates were conducted to characterize the cognate antigens. Nine of 12 sera reacted with proteins in the range of 162-180 kDa, three of which were found to react specifically with the 162 kDa 35S methionine labeled recombinant early endosome antigen 1. These proteins were not associated with common RNA. Although complete clinical information was not available, some of the patients had rheumatoid arthritis(RA). In addition, the RNA-IPP results suggest that other patients included one each with SLE, SSc, polymyositis, and Sjögren's syndrome. Anti-early endosome antigen 1 antibody was found in 25%(3 of 12) of sera known to stain cytoplasmic vesicles. The reactive sera came mostly from patients with RA. The sera was from one case each of clinical-confirmed RA, SLE and Sjögren's syndrome.

UR - http://www.scopus.com/inward/record.url?scp=0141957965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141957965&partnerID=8YFLogxK

M3 - Article

VL - 51

SP - 501

EP - 507

JO - Rinsho byori. The Japanese journal of clinical pathology

JF - Rinsho byori. The Japanese journal of clinical pathology

SN - 0047-1860

IS - 6

ER -