Detection of brain-specific gene expression in brain cells in primary culture

A novel promoter assay based on the use of a retrovirus vector

K. Ikenaka, K. Nakahira, Kazunori Nakajima, I. Fujimoto, T. Kagawa, M. Ogawa, K. Mikoshiba

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Promoter activities of the brain-specific genes for glial fibrillary acidic protein (GFAP) and myelin basic (MBP) were investigated in brain cells in primary culture with the use of a novel retrovirus vector, pIP200. With this vector, promoter activity can be expressed in terms of β-galactosidase activity. Differentiation of the primary brain cells to mature glial cells was not affected by treatment with the pIP200 virus vector. The 256-bp 5'-flanking region of the GFAP gene directed astrocyte-specific expression of lacZ. It was silent in fibroblasts, even in multiple copies. The 1.3-kb 5'-flanking region of the MBP gene exhibited strict tissue (oligodendrocyte) specificity under the present assay method but showed some leakiness when integrated into the chromosome in multiple copies. Promoter regions conferring cell type specificity in brain were effectively identified by the present method.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalNew Biologist
Volume4
Issue number1
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Primary Cell Culture
Retroviridae
Gene expression
Assays
Brain
Gene Expression
Genes
5' Flanking Region
Glial Fibrillary Acidic Protein
Galactosidases
Organ Specificity
Oligodendroglia
Fibroblasts
Chromosomes
Myelin Sheath
Viruses
Genetic Promoter Regions
Neuroglia
Astrocytes
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Detection of brain-specific gene expression in brain cells in primary culture : A novel promoter assay based on the use of a retrovirus vector. / Ikenaka, K.; Nakahira, K.; Nakajima, Kazunori; Fujimoto, I.; Kagawa, T.; Ogawa, M.; Mikoshiba, K.

In: New Biologist, Vol. 4, No. 1, 1992, p. 53-60.

Research output: Contribution to journalArticle

Ikenaka, K. ; Nakahira, K. ; Nakajima, Kazunori ; Fujimoto, I. ; Kagawa, T. ; Ogawa, M. ; Mikoshiba, K. / Detection of brain-specific gene expression in brain cells in primary culture : A novel promoter assay based on the use of a retrovirus vector. In: New Biologist. 1992 ; Vol. 4, No. 1. pp. 53-60.
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