Detection of Campylobacter rectus in periodontitis sites by monoclonal antibodies

Hideaki Ihara, Tadashi Miura, Tetsuo Kato, Kazuyuki Ishihara, Taneaki Nakagawa, Satoru Yamada, Katsuji Okuda

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Campylobacter rectus, a gram-negative, microaerophilic, and motile bacterium, has been proposed to play a pathogenic role in human periodontitis. Surface components, such as the flagellum, surface layer (S-layer), and cytotoxin, have been reported as possible virulence factors of the microorganism. In the present study, monoclonal antibodies against surface components of this bacterium were produced to detect and investigate the pathogenic potential of C. rectus in periodontitis. Two monoclonal antibodies, designated CRT-1 and CRT-2, recognized a peculiar 150 kDa S-layer protein by immunoblot analysis. The CRT-2 antibody reacted to all C. rectus strains tested, except for the S-layer negative strain of the species [C. rectus ATCC 33238 S-layer (-) strain]. The CRT-3 antibody reacted to a 60-kDa protein in C. rectus and also cross-reacted with Campylobacter showae ATCC 51164 and CCUG 11641 strains. Using the dot-blot method, we were able to detect C. rectus using the CRT-2 antibody when as few as 103 organisms were present in a subgingival dental plaque sample. Detection of C. rectus in plaque samples correlated significantly with clinical findings such as probing depth (P < 0.001), bleeding on probing (P < 0.001), and gingival index (P < 0.001). These findings indicate that infection by C. rectus may be an important indicator of periodontal disease status.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalJournal of Periodontal Research
Volume38
Issue number1
DOIs
Publication statusPublished - 2003 Feb
Externally publishedYes

Fingerprint

Campylobacter rectus
Periodontitis
Monoclonal Antibodies
Antibodies
Bacteria
Periodontal Index
Dental Plaque
Campylobacter
Flagella
Cytotoxins
Periodontal Diseases
Virulence Factors
Protein C
Hemorrhage

Keywords

  • Campylobacter rectus
  • Dot-blot analysis
  • Monoclonal antibody
  • Periodontal sites

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Detection of Campylobacter rectus in periodontitis sites by monoclonal antibodies. / Ihara, Hideaki; Miura, Tadashi; Kato, Tetsuo; Ishihara, Kazuyuki; Nakagawa, Taneaki; Yamada, Satoru; Okuda, Katsuji.

In: Journal of Periodontal Research, Vol. 38, No. 1, 02.2003, p. 64-72.

Research output: Contribution to journalArticle

Ihara, Hideaki ; Miura, Tadashi ; Kato, Tetsuo ; Ishihara, Kazuyuki ; Nakagawa, Taneaki ; Yamada, Satoru ; Okuda, Katsuji. / Detection of Campylobacter rectus in periodontitis sites by monoclonal antibodies. In: Journal of Periodontal Research. 2003 ; Vol. 38, No. 1. pp. 64-72.
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