Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry

Hyun Jeong Yang, Itsuko Ishizaki, Noriaki Sanada, Nobuhiro Zaima, Yuki Sugiura, Ikuko Yao, Koji Ikegami, Mitsutoshi Setou

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalMedical Molecular Morphology
Volume43
Issue number3
DOIs
Publication statusPublished - 2010 Sep
Externally publishedYes

Fingerprint

Secondary Ion Mass Spectrometry
Neurites
Phospholipids
Fatty Acids
Ions
Lipids
Sphingomyelins
Carisoprodol
Phosphatidylcholines
Palmitic Acids
Oleic Acids
Superior Cervical Ganglion
Phosphorylcholine
Lipid Bilayers
Research
Cell Biology
Mass Spectrometry
Central Nervous System
Neurons

Keywords

  • Fatty acid
  • Imaging
  • Neurite
  • Neuron
  • Phospholipid
  • TOF-SIMS

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry. / Yang, Hyun Jeong; Ishizaki, Itsuko; Sanada, Noriaki; Zaima, Nobuhiro; Sugiura, Yuki; Yao, Ikuko; Ikegami, Koji; Setou, Mitsutoshi.

In: Medical Molecular Morphology, Vol. 43, No. 3, 09.2010, p. 158-164.

Research output: Contribution to journalArticle

Yang, Hyun Jeong ; Ishizaki, Itsuko ; Sanada, Noriaki ; Zaima, Nobuhiro ; Sugiura, Yuki ; Yao, Ikuko ; Ikegami, Koji ; Setou, Mitsutoshi. / Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry. In: Medical Molecular Morphology. 2010 ; Vol. 43, No. 3. pp. 158-164.
@article{a6d8c56cc7e949c4914171177f141448,
title = "Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry",
abstract = "Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.",
keywords = "Fatty acid, Imaging, Neurite, Neuron, Phospholipid, TOF-SIMS",
author = "Yang, {Hyun Jeong} and Itsuko Ishizaki and Noriaki Sanada and Nobuhiro Zaima and Yuki Sugiura and Ikuko Yao and Koji Ikegami and Mitsutoshi Setou",
year = "2010",
month = "9",
doi = "10.1007/s00795-009-0487-2",
language = "English",
volume = "43",
pages = "158--164",
journal = "Medical Molecular Morphology",
issn = "1860-1480",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry

AU - Yang, Hyun Jeong

AU - Ishizaki, Itsuko

AU - Sanada, Noriaki

AU - Zaima, Nobuhiro

AU - Sugiura, Yuki

AU - Yao, Ikuko

AU - Ikegami, Koji

AU - Setou, Mitsutoshi

PY - 2010/9

Y1 - 2010/9

N2 - Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.

AB - Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.

KW - Fatty acid

KW - Imaging

KW - Neurite

KW - Neuron

KW - Phospholipid

KW - TOF-SIMS

UR - http://www.scopus.com/inward/record.url?scp=77956280542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956280542&partnerID=8YFLogxK

U2 - 10.1007/s00795-009-0487-2

DO - 10.1007/s00795-009-0487-2

M3 - Article

C2 - 20857264

AN - SCOPUS:77956280542

VL - 43

SP - 158

EP - 164

JO - Medical Molecular Morphology

JF - Medical Molecular Morphology

SN - 1860-1480

IS - 3

ER -