Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro

Kou Sueoka, A. M. Dharmarajan, E. Michael, S. J. Atlas, E. E. Wallach

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two peaks of rabbit serum EPF activity were seen over the course of pregnancy. Rabbit ovaries with or without attached oviducts were perfused in vitro for 5 h beginning 12, 16, 24, 48, 72 and 120 h after mating. Perfused isolated ovaries did not produce EPF in vitro, but significant EPF activity was detected in the perfusate of the ovary together with oviduct. Pseudopregnant animals and those rabbits that did not ovulate exhibited no perfusate EPF activity. Perfusate EPF activity was highest at the time embryos were at the pronuclear stage and continued through the morula stage. Although the location of embryos at 72 h after mating varied between oviduct and uterus, EPF activity was maintained over the perfusion period. The results suggest that EPF release occurs within 3 h of fertilization and that the presence of the preblastocyst embryo is crucial for EPF release.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalJournal of Reproduction and Fertility
Volume84
Issue number1
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Oviducts
Ovary
Rabbits
Embryonic Structures
Morula
In Vitro Techniques
early pregnancy factor
Fertilization
Uterus
Perfusion
Pregnancy
Serum

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Embryology
  • Obstetrics and Gynaecology

Cite this

Sueoka, K., Dharmarajan, A. M., Michael, E., Atlas, S. J., & Wallach, E. E. (1988). Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro. Journal of Reproduction and Fertility, 84(1), 325-331.

Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro. / Sueoka, Kou; Dharmarajan, A. M.; Michael, E.; Atlas, S. J.; Wallach, E. E.

In: Journal of Reproduction and Fertility, Vol. 84, No. 1, 1988, p. 325-331.

Research output: Contribution to journalArticle

Sueoka, K, Dharmarajan, AM, Michael, E, Atlas, SJ & Wallach, EE 1988, 'Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro', Journal of Reproduction and Fertility, vol. 84, no. 1, pp. 325-331.
Sueoka, Kou ; Dharmarajan, A. M. ; Michael, E. ; Atlas, S. J. ; Wallach, E. E. / Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro. In: Journal of Reproduction and Fertility. 1988 ; Vol. 84, No. 1. pp. 325-331.
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