Detection of endometrial cancer by flow cytometry using two monoclonal antibodies

Koji Kobiki, Katsumi Tsukasaki, Kaneyuki Kubushiro, Yoichi Kobayashi, Kiyoshi Hasegawa, Takeshi Fukuchi, Koji Banno, Shiro Nozawa

Research output: Contribution to journalArticle

Abstract

Background: To develop a supplementary diagnostic method for endometrial cancer by measuring the reactivity of various endometrial lesions with two monoclonal antibodies. Methods: We investigated the reactivity of various endometrial lesions with two monoclonal antibodies (MSN-1 and MSN-3) by flow cytometry (one-color and two-color methods). Results: The two-color method appeared to be suitable for use in place of simultaneous performance of the one-color methods with MSN-1 and MSN-3. The positivity rate for normal endometrium was 16.0% with the two-color method, which was lower than the rate of 30.0% obtained with concomitant used of the one-color methods. The positivity rate for endometrial cancer was high, 84.0%, with the two-color method. The positivity rate was 85.7% for well-differentiated endometrial cancer, 71.4% for moderately differentiated cancer, and 100.0% for poorly differentiated cancer; thus, the rate was high irrespective of the cellular differentiation. Conclusions: The two-color method is more useful than the one-color method as a supplementary diagnostic procedure for endometrial cancer.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalCytometry
Volume36
Issue number2
DOIs
Publication statusPublished - 1999 Jun 1

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Keywords

  • Diagnostic method
  • Endometrial cancer
  • Flow cytometry
  • MSN-1
  • MSN-3

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

Cite this

Kobiki, K., Tsukasaki, K., Kubushiro, K., Kobayashi, Y., Hasegawa, K., Fukuchi, T., Banno, K., & Nozawa, S. (1999). Detection of endometrial cancer by flow cytometry using two monoclonal antibodies. Cytometry, 36(2), 150-156. https://doi.org/10.1002/(SICI)1097-0320(19990601)36:2<150::AID-CYTO9>3.0.CO;2-J