Detection of heat shock protein in patients with insulin-dependent diabetes mellitus

Izumi Takei, Tomohiro Kasatani, Kenji Watanabe, Akira Kasuga, Akira Shimada, Taro Maruyama, Takao Saruta, Hiroko Nomaguchi

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Abstract

We have investigated whether antibodies to heat shock protein (hsp) 65 are present in sera from patients with insulin-dependent diabetes mellitus by using Mycobacterium leprae hsp65. Fifty-two sera from patients with IDDM, 36 from patients with unclassified insulin-treated diabetes mellitus and 41 from normal healthy controls were examined by ELISA assay. Seventeen (32.7%) out of 52 IDDM sera and 10 (27.8%) out of unclassified insulin-treated diabetic sera were positive for anti-Mycobacterium (anti-M. leprae) hsp65 antibodies while none of the healthy control sera were positive. Based on western blot analysis, 12 of the 17 IDDM sera and 1 of 2 sera from the unclassified insulin-treated diabetics were positive for anti-M. leprae hsp65 antibodies while all normal control sera were negative. These results support the idea that hsp65 may play a role in the pathogenesis of IDDM. Future studies are necessary to elucidate the role of hsp65 in the pathogenesis of IDDM.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalDiabetes Research and Clinical Practice
Volume21
Issue number1
DOIs
Publication statusPublished - 1993 Jul

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Keywords

  • Heat shock protein
  • Immunology
  • Insulin-dependent diabetes mellitus (IDDM)
  • Mycobacterium leprae

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Takei, I., Kasatani, T., Watanabe, K., Kasuga, A., Shimada, A., Maruyama, T., Saruta, T., & Nomaguchi, H. (1993). Detection of heat shock protein in patients with insulin-dependent diabetes mellitus. Diabetes Research and Clinical Practice, 21(1), 25-29. https://doi.org/10.1016/0168-8227(93)90093-K