Detection of HEY1-NCOA2 fusion by fluorescence in-situ hybridization in formalin-fixed paraffin-embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma

Turrent Robert Nakayama, Yasuhiro Miura, Jiro Ogino, Michiro Susa, Itsuo Watanabe, Keisuke Horiuchi, Ukei Anazawa, Yoshiaki Toyama, Hideo Morioka, Makio Mukai, Tadashi Hasegawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Mesenchymal chondrosarcoma (MC) is an extremely rare subtype of chondrosarcoma. A tumor specific fusion gene, HEY1-NCOA2 fusion, was recently identified in this tumor. The finding raises the possibility that the diagnosis of MC can be improved by examining the fusion gene. In the present study, we aimed to evaluate the efficacy of fluorescence in situ hybridization (FISH) in detecting HEY1-NCOA2 fusion for the diagnosis of MC. Specimens from 10 patients diagnosed with MC were used for the study. Dual-color FISH was performed using two different probes that specifically hybridize to HEY1 and NCOA2, respectively. Fusion signals were identified in all but two specimens, in which no signal was detected, presumably because of inadequate sample preparation. In accordance with results of a previous study, FISH analysis was highly sensitive in detecting HEY1-NCOA2 fusion in adequately prepared MC samples. The current study adds further support for the use of HEY1-NCOA2 fusion as a valid diagnostic marker for MC.

Original languageEnglish
Pages (from-to)823-826
Number of pages4
JournalPathology International
Volume62
Issue number12
DOIs
Publication statusPublished - 2012 Dec

Fingerprint

Mesenchymal Chondrosarcoma
Fluorescence In Situ Hybridization
Paraffin
Formaldehyde
Gene Fusion
Chondrosarcoma
Neoplasms
Color

Keywords

  • Fluorescence in situ hybridization
  • Fusion gene
  • Mesenchymal chondrosarcoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of HEY1-NCOA2 fusion by fluorescence in-situ hybridization in formalin-fixed paraffin-embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma. / Nakayama, Turrent Robert; Miura, Yasuhiro; Ogino, Jiro; Susa, Michiro; Watanabe, Itsuo; Horiuchi, Keisuke; Anazawa, Ukei; Toyama, Yoshiaki; Morioka, Hideo; Mukai, Makio; Hasegawa, Tadashi.

In: Pathology International, Vol. 62, No. 12, 12.2012, p. 823-826.

Research output: Contribution to journalArticle

Nakayama, Turrent Robert ; Miura, Yasuhiro ; Ogino, Jiro ; Susa, Michiro ; Watanabe, Itsuo ; Horiuchi, Keisuke ; Anazawa, Ukei ; Toyama, Yoshiaki ; Morioka, Hideo ; Mukai, Makio ; Hasegawa, Tadashi. / Detection of HEY1-NCOA2 fusion by fluorescence in-situ hybridization in formalin-fixed paraffin-embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma. In: Pathology International. 2012 ; Vol. 62, No. 12. pp. 823-826.
@article{fd085fa8078e4f2b93c38328b9d57f38,
title = "Detection of HEY1-NCOA2 fusion by fluorescence in-situ hybridization in formalin-fixed paraffin-embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma",
abstract = "Mesenchymal chondrosarcoma (MC) is an extremely rare subtype of chondrosarcoma. A tumor specific fusion gene, HEY1-NCOA2 fusion, was recently identified in this tumor. The finding raises the possibility that the diagnosis of MC can be improved by examining the fusion gene. In the present study, we aimed to evaluate the efficacy of fluorescence in situ hybridization (FISH) in detecting HEY1-NCOA2 fusion for the diagnosis of MC. Specimens from 10 patients diagnosed with MC were used for the study. Dual-color FISH was performed using two different probes that specifically hybridize to HEY1 and NCOA2, respectively. Fusion signals were identified in all but two specimens, in which no signal was detected, presumably because of inadequate sample preparation. In accordance with results of a previous study, FISH analysis was highly sensitive in detecting HEY1-NCOA2 fusion in adequately prepared MC samples. The current study adds further support for the use of HEY1-NCOA2 fusion as a valid diagnostic marker for MC.",
keywords = "Fluorescence in situ hybridization, Fusion gene, Mesenchymal chondrosarcoma",
author = "Nakayama, {Turrent Robert} and Yasuhiro Miura and Jiro Ogino and Michiro Susa and Itsuo Watanabe and Keisuke Horiuchi and Ukei Anazawa and Yoshiaki Toyama and Hideo Morioka and Makio Mukai and Tadashi Hasegawa",
year = "2012",
month = "12",
doi = "10.1111/pin.12022",
language = "English",
volume = "62",
pages = "823--826",
journal = "Pathology International",
issn = "1320-5463",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Detection of HEY1-NCOA2 fusion by fluorescence in-situ hybridization in formalin-fixed paraffin-embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma

AU - Nakayama, Turrent Robert

AU - Miura, Yasuhiro

AU - Ogino, Jiro

AU - Susa, Michiro

AU - Watanabe, Itsuo

AU - Horiuchi, Keisuke

AU - Anazawa, Ukei

AU - Toyama, Yoshiaki

AU - Morioka, Hideo

AU - Mukai, Makio

AU - Hasegawa, Tadashi

PY - 2012/12

Y1 - 2012/12

N2 - Mesenchymal chondrosarcoma (MC) is an extremely rare subtype of chondrosarcoma. A tumor specific fusion gene, HEY1-NCOA2 fusion, was recently identified in this tumor. The finding raises the possibility that the diagnosis of MC can be improved by examining the fusion gene. In the present study, we aimed to evaluate the efficacy of fluorescence in situ hybridization (FISH) in detecting HEY1-NCOA2 fusion for the diagnosis of MC. Specimens from 10 patients diagnosed with MC were used for the study. Dual-color FISH was performed using two different probes that specifically hybridize to HEY1 and NCOA2, respectively. Fusion signals were identified in all but two specimens, in which no signal was detected, presumably because of inadequate sample preparation. In accordance with results of a previous study, FISH analysis was highly sensitive in detecting HEY1-NCOA2 fusion in adequately prepared MC samples. The current study adds further support for the use of HEY1-NCOA2 fusion as a valid diagnostic marker for MC.

AB - Mesenchymal chondrosarcoma (MC) is an extremely rare subtype of chondrosarcoma. A tumor specific fusion gene, HEY1-NCOA2 fusion, was recently identified in this tumor. The finding raises the possibility that the diagnosis of MC can be improved by examining the fusion gene. In the present study, we aimed to evaluate the efficacy of fluorescence in situ hybridization (FISH) in detecting HEY1-NCOA2 fusion for the diagnosis of MC. Specimens from 10 patients diagnosed with MC were used for the study. Dual-color FISH was performed using two different probes that specifically hybridize to HEY1 and NCOA2, respectively. Fusion signals were identified in all but two specimens, in which no signal was detected, presumably because of inadequate sample preparation. In accordance with results of a previous study, FISH analysis was highly sensitive in detecting HEY1-NCOA2 fusion in adequately prepared MC samples. The current study adds further support for the use of HEY1-NCOA2 fusion as a valid diagnostic marker for MC.

KW - Fluorescence in situ hybridization

KW - Fusion gene

KW - Mesenchymal chondrosarcoma

UR - http://www.scopus.com/inward/record.url?scp=84871366771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871366771&partnerID=8YFLogxK

U2 - 10.1111/pin.12022

DO - 10.1111/pin.12022

M3 - Article

VL - 62

SP - 823

EP - 826

JO - Pathology International

JF - Pathology International

SN - 1320-5463

IS - 12

ER -