Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

Teruhiko Matsubara; Ayaka Ogami; Haruka Kori; Mineo Hashizume; Toshinori Sato

doi:10.1021/acsabm.2c00276

Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

Teruhiko Matsubara, Ayaka Ogami, Haruka Kori, Mineo Hashizume, Toshinori Sato

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

Abstract

The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

Original language	English
Journal	ACS Applied Bio Materials
DOIs	https://doi.org/10.1021/acsabm.2c00276
Publication status	Accepted/In press - 2022

Keywords

hemagglutination
influenza virus
polystyrene microparticle
red blood cell
saccharide primer
sialyl oligosaccharide

ASJC Scopus subject areas

Biomaterials
Chemistry(all)
Biomedical Engineering
Biochemistry, medical

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10.1021/acsabm.2c00276

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@article{0745ac4d8904410f9bfa9d4ee301c31e,

title = "Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells",

abstract = "The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.",

keywords = "hemagglutination, influenza virus, polystyrene microparticle, red blood cell, saccharide primer, sialyl oligosaccharide",

author = "Teruhiko Matsubara and Ayaka Ogami and Haruka Kori and Mineo Hashizume and Toshinori Sato",

note = "Funding Information: This work was funded by the Izumi Science and Technology Foundation (Osaka, Japan) (grant no. 2021-J-051) to T.M. Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",

year = "2022",

doi = "10.1021/acsabm.2c00276",

language = "English",

journal = "ACS Applied Bio Materials",

issn = "2576-6422",

publisher = "American Chemical Society",

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T1 - Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

AU - Matsubara, Teruhiko

AU - Ogami, Ayaka

AU - Kori, Haruka

AU - Hashizume, Mineo

AU - Sato, Toshinori

PY - 2022

Y1 - 2022

N2 - The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

AB - The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

KW - hemagglutination

KW - influenza virus

KW - polystyrene microparticle

KW - red blood cell

KW - saccharide primer

KW - sialyl oligosaccharide

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Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

Abstract

Keywords

ASJC Scopus subject areas

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