Abstract
Abstract: Influenza virus (IFV) detection in the early phase of disease is critical for effective anti-influenza therapy using neuraminidase inhibitors. Sialyloligosaccharide receptors on the surface of respiratory cells are recognized by IFV hemagglutinin (HA) in the infection. Here, we show that agglutination of IFV is detected using poly(glycidyl methacrylate) (PGMA)-coated polystyrene nanoparticles conjugated with a sialic acid-mimic peptide. The azido peptide was immobilized onto the surface of the PGMA-coated nanoparticles by click chemistry. The distribution of particle size, determined by dynamic light scattering, indicated that the peptide-conjugated nanoparticles were agglutinated in the presence of HA and IFV. Nanoparticles conjugated with the receptor-mimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza.
| Original language | English |
|---|---|
| Journal | Polymer Journal |
| DOIs | |
| Publication status | Accepted/In press - 2019 Jan 1 |
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ASJC Scopus subject areas
- Polymers and Plastics
- Materials Chemistry
Cite this
Detection of influenza virus by agglutination using nanoparticles conjugated with a sialic acid-mimic peptide. / Matsubara, Teruhiko; Kubo, Akane; Sato, Toshinori.
In: Polymer Journal, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of influenza virus by agglutination using nanoparticles conjugated with a sialic acid-mimic peptide
AU - Matsubara, Teruhiko
AU - Kubo, Akane
AU - Sato, Toshinori
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Abstract: Influenza virus (IFV) detection in the early phase of disease is critical for effective anti-influenza therapy using neuraminidase inhibitors. Sialyloligosaccharide receptors on the surface of respiratory cells are recognized by IFV hemagglutinin (HA) in the infection. Here, we show that agglutination of IFV is detected using poly(glycidyl methacrylate) (PGMA)-coated polystyrene nanoparticles conjugated with a sialic acid-mimic peptide. The azido peptide was immobilized onto the surface of the PGMA-coated nanoparticles by click chemistry. The distribution of particle size, determined by dynamic light scattering, indicated that the peptide-conjugated nanoparticles were agglutinated in the presence of HA and IFV. Nanoparticles conjugated with the receptor-mimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza.
AB - Abstract: Influenza virus (IFV) detection in the early phase of disease is critical for effective anti-influenza therapy using neuraminidase inhibitors. Sialyloligosaccharide receptors on the surface of respiratory cells are recognized by IFV hemagglutinin (HA) in the infection. Here, we show that agglutination of IFV is detected using poly(glycidyl methacrylate) (PGMA)-coated polystyrene nanoparticles conjugated with a sialic acid-mimic peptide. The azido peptide was immobilized onto the surface of the PGMA-coated nanoparticles by click chemistry. The distribution of particle size, determined by dynamic light scattering, indicated that the peptide-conjugated nanoparticles were agglutinated in the presence of HA and IFV. Nanoparticles conjugated with the receptor-mimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza.
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UR - http://www.scopus.com/inward/citedby.url?scp=85073953129&partnerID=8YFLogxK
U2 - 10.1038/s41428-019-0252-x
DO - 10.1038/s41428-019-0252-x
M3 - Article
AN - SCOPUS:85073953129
JO - Polymer Journal
JF - Polymer Journal
SN - 0032-3896
ER -