Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells

Takashi Tsuruo, Yoshikazu Sugimoto, Hirofumi Hamada, Igor Roninson, Masao Okumura, Koichi Adachi, Yasuo Morishima, Ryuzo Ohno

Research output: Contribution to journalArticle

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Abstract

We have examined the expression of P-glycoprotein in clinical leukemic cell samples by using a monoclonal antibody (MRK16) against P-glycoprotein. We found that leukemia cells isolated from 3 out of 6 patients with blast crisis of chronic myelogenous leukemia were reactive to MRK16. These 3 cell lines expressed high levels of mdr1 mRNA, which codes for P-glycoprotein. The present result indicates that the clinically refractory state of the tumor may be predicted in part by determining P-glycoprotein expression using the monoclonal antibody against P-glycoprotein, and the mdr1 probe.

Original languageEnglish
Pages (from-to)1415-1419
Number of pages5
JournalJapanese Journal of Cancer Research
Volume78
Issue number12
Publication statusPublished - 1987 Dec
Externally publishedYes

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Multiple Drug Resistance
P-Glycoprotein
Leukemia
Messenger RNA
Monoclonal Antibodies
Blast Crisis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Cell Line
Neoplasms

Keywords

  • Drug resistance
  • Drug resistance gene
  • Gene amplification
  • Gene expression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Tsuruo, T., Sugimoto, Y., Hamada, H., Roninson, I., Okumura, M., Adachi, K., ... Ohno, R. (1987). Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells. Japanese Journal of Cancer Research, 78(12), 1415-1419.

Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells. / Tsuruo, Takashi; Sugimoto, Yoshikazu; Hamada, Hirofumi; Roninson, Igor; Okumura, Masao; Adachi, Koichi; Morishima, Yasuo; Ohno, Ryuzo.

In: Japanese Journal of Cancer Research, Vol. 78, No. 12, 12.1987, p. 1415-1419.

Research output: Contribution to journalArticle

Tsuruo, T, Sugimoto, Y, Hamada, H, Roninson, I, Okumura, M, Adachi, K, Morishima, Y & Ohno, R 1987, 'Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells', Japanese Journal of Cancer Research, vol. 78, no. 12, pp. 1415-1419.
Tsuruo, Takashi ; Sugimoto, Yoshikazu ; Hamada, Hirofumi ; Roninson, Igor ; Okumura, Masao ; Adachi, Koichi ; Morishima, Yasuo ; Ohno, Ryuzo. / Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells. In: Japanese Journal of Cancer Research. 1987 ; Vol. 78, No. 12. pp. 1415-1419.
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