Detection of Multidrug Resistance Markers, P-Glycoprotein and mdrl mRNA, in Human Leukemia Cells

Takashi Tsuruo, Yoshikazu Sugimoto, Hirofumi Hamada, Igor Roninson, Masao Okumura, Koichi Adachi, Yasuo Morishima, Ryuzo Ohno

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Abstract

We have examined the expression of P-glycoprotein in clinical leukemic cell samples by using a monoclonal antibody (MRK16) against P-glycoprotein. We found that leukemia cells isolated from 3 out of 6 patients with blast crisis of chronic myelogenous leukemia were reactive to MRK16. These 3 cell lines expressed high levels of mdrl mRNA, which codes for P-glycoprotein. The present result indicates that the clinically refractory state of the tumor may be predicted in part by determining P-glycoprotein expression using the monoclonal antibody against P-glycoprotein, and the mdrl probe.

Original languageEnglish
Pages (from-to)1415-1419
Number of pages5
JournalJapanese Journal of Cancer Research GANN
Volume78
Issue number12
Publication statusPublished - 1987 Jan 1
Externally publishedYes

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Keywords

  • Drug resistance
  • Drug resistance gene
  • Gene amplification
  • Gene expression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Tsuruo, T., Sugimoto, Y., Hamada, H., Roninson, I., Okumura, M., Adachi, K., Morishima, Y., & Ohno, R. (1987). Detection of Multidrug Resistance Markers, P-Glycoprotein and mdrl mRNA, in Human Leukemia Cells. Japanese Journal of Cancer Research GANN, 78(12), 1415-1419.