Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

Hiroki Ito, Takuya Terai, Kenjiro Hanaoka, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities. This journal is

Original languageEnglish
Pages (from-to)8319-8322
Number of pages4
JournalChemical Communications
Volume51
Issue number39
DOIs
Publication statusPublished - 2015 May 14
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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