Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

Hiroki Ito; Takuya Terai; Kenjiro Hanaoka; Tasuku Ueno; Toru Komatsu; Tetsuo Nagano; Yasuteru Urano

doi:10.1039/c5cc01613d

Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

Hiroki Ito, Takuya Terai, Kenjiro Hanaoka, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano, Yasuteru Urano

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities. This journal is

Original language	English
Pages (from-to)	8319-8322
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	39
DOIs	https://doi.org/10.1039/c5cc01613d
Publication status	Published - 2015 May 14
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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AU - Ito, Hiroki

AU - Terai, Takuya

AU - Hanaoka, Kenjiro

AU - Ueno, Tasuku

AU - Komatsu, Toru

AU - Nagano, Tetsuo

AU - Urano, Yasuteru

N1 - Publisher Copyright: © The Royal Society of Chemistry 2015.

PY - 2015/5/14

Y1 - 2015/5/14

N2 - We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities. This journal is

AB - We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities. This journal is

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JO - Chemical Communications

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Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

Abstract

ASJC Scopus subject areas

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