Detection of numerical and structural alterations and fusion of chromosomes 16 and 1 in low-grade papillary breast carcinoma by fluorescence in situ hybridization

Hitoshi Tsuda, Teruko Takarabe, Nobuyuki Susumu, Johji Inazawa, Shuichi Okada, Setsuo Hirohashi

Research output: Contribution to journalArticle

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Abstract

Intracystic papillary breast tumors, including intraductal papilloma and low-grade intracystic papillary carcinoma, constitute a group for which differential diagnosis is frequently difficult. We examined the status of chromosomes 16 and 1 by multicolor fluorescence in situ hybridization (FISH) analyses and the DNA ploidy patterns by flow cytometry in 26 intracystic papillary tumors. Alterations of chromosomes 16 and 1 were detected by FISH in 93 and 85%, respectively, of 14 low-grade papillary carcinomas, and the latter alterations always concurred with the former. Two-color FISH using probes for the DIZ1 (1q12) and D16Z2 (16cen) loci or the DIZ1 and D16Z3 (16q11) loci showed that fusion of chromosomes 16 and 1, mostly with breakpoints distal to 16q11.2 and proximal to 1q12, occurred in 77% of the papillary carcinomas. DNA aneuploidy was detected in 6% of these carcinomas. No papilloma showed these chromosome alterations or DNA aneuploidy. Chromosome 16 and 1 fusions appeared to occur frequently in diploid breast carcinomas and to be involved in the acquisition of a malignant phenotype by duct epithelial cells. We suggest that two-color FISH methods for detecting 1;16 fusions might be applicable as supportive methods for the differential diagnosis of intracystic papillary breast tumors.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalAmerican Journal of Pathology
Volume151
Issue number4
Publication statusPublished - 1997 Oct
Externally publishedYes

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Chromosomes, Human, Pair 16
Papillary Carcinoma
Chromosomes, Human, Pair 1
Fluorescence In Situ Hybridization
Aneuploidy
Breast Neoplasms
DNA
Differential Diagnosis
Intraductal Papilloma
Color
Ploidies
Papilloma
Diploidy
Flow Cytometry
Chromosomes
Epithelial Cells
Carcinoma
Phenotype
Breast Carcinoma In Situ
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of numerical and structural alterations and fusion of chromosomes 16 and 1 in low-grade papillary breast carcinoma by fluorescence in situ hybridization. / Tsuda, Hitoshi; Takarabe, Teruko; Susumu, Nobuyuki; Inazawa, Johji; Okada, Shuichi; Hirohashi, Setsuo.

In: American Journal of Pathology, Vol. 151, No. 4, 10.1997, p. 1027-1034.

Research output: Contribution to journalArticle

Tsuda, Hitoshi ; Takarabe, Teruko ; Susumu, Nobuyuki ; Inazawa, Johji ; Okada, Shuichi ; Hirohashi, Setsuo. / Detection of numerical and structural alterations and fusion of chromosomes 16 and 1 in low-grade papillary breast carcinoma by fluorescence in situ hybridization. In: American Journal of Pathology. 1997 ; Vol. 151, No. 4. pp. 1027-1034.
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