Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction

H. Tachibana, S. Kobayashi, E. Okuzawa, G. Masuda

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Tachibana H., Kobayashi S., Okuzawa E. and Masuda G. 1992. Detection of pathogenic Entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. International Journal for Parasitology 22: 1193-1196. A sensitive method for detection of pathogenic Entamoeba histolytica DNA in drained fluids from liver abscess patients, using the polymerase chain reaction (PCR), has been developed. The PCR employs oligonucleotide primers specific for the gene encoding the 30 kDa molecule of pathogenic E. histolytica. Liver abscess fluids (19 samples), from 14 patients with a presumptive amebic liver abscess, were examined microscopically and by the PCR method. Only two of the 19 samples were positive microscopically, whereas all 19 samples tested positive by PCR. This technique can be used to confirm the diagnosis of amebic liver abscess.

Original languageEnglish
Pages (from-to)1193-1196
Number of pages4
JournalInternational Journal for Parasitology
Volume22
Issue number8
DOIs
Publication statusPublished - 1992

Fingerprint

Liver Abscess
Entamoeba histolytica
Polymerase Chain Reaction
DNA
Amoebic Liver Abscess
Parasitology
DNA Primers
Genes

Keywords

  • amebic liver abscess
  • DNA
  • Entamoeba histolytica
  • polymerase chain reaction

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. / Tachibana, H.; Kobayashi, S.; Okuzawa, E.; Masuda, G.

In: International Journal for Parasitology, Vol. 22, No. 8, 1992, p. 1193-1196.

Research output: Contribution to journalArticle

Tachibana, H. ; Kobayashi, S. ; Okuzawa, E. ; Masuda, G. / Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction. In: International Journal for Parasitology. 1992 ; Vol. 22, No. 8. pp. 1193-1196.
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