Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation

Tamotsu Ebihara, Takashi Hashimoto, Jun Kudo, Shinobu Gamou, Nobuyoshi Shimizu, Takeji Nishikawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

There has been controversy concerning the nature of the bullous pemphigoid (BP) antigen: immunoprecipitation identified BP antigen as a single, unique 230-kDa protein, whereas immunoblot analysis showed multiple antigen molecules, mainly 230- and 170-kDa proteins. In this study, to further characterize the 170-kDa protein, we have examined whether the 170-kDa protein is detected by immunoprecipitation. Extracts of human squamous cell carcinoma cells revealed the 170-kDa protein with immunoblot analysis. Although the conventional immunoprecipitation detected only the 230-kDa protein, some BP sera that detected the 170-kDa protein with immunoblotting also precipitated the 170-kDa protein with our modified immunoprecipitation, in which the cells were extracted with 1% sodium dodecylsulfate (SDS) buffer and reacted with the sera under reduced SDS concentration. The 170-kDa protein-specific BP sera clearly showed hemidesmosomal plaque staining with immunofluorescence of cultured cells. These results indicate that the 170-kDa protein is indeed one of the BP antigens and that the 230- and 170-kDa BP antigens are integrated in different ways in hemidesmosomes.

Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalJournal of Investigative Dermatology
Volume100
Issue number2
Publication statusPublished - 1993 Feb

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Bullous Pemphigoid
Immunoprecipitation
Antigens
Proteins
Sodium
Serum
Cells
Hemidesmosomes
Immunoblotting
Fluorescent Antibody Technique
Squamous Cell Carcinoma
Cultured Cells
Buffers
Staining and Labeling

ASJC Scopus subject areas

  • Dermatology

Cite this

Ebihara, T., Hashimoto, T., Kudo, J., Gamou, S., Shimizu, N., & Nishikawa, T. (1993). Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation. Journal of Investigative Dermatology, 100(2), 176-179.

Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation. / Ebihara, Tamotsu; Hashimoto, Takashi; Kudo, Jun; Gamou, Shinobu; Shimizu, Nobuyoshi; Nishikawa, Takeji.

In: Journal of Investigative Dermatology, Vol. 100, No. 2, 02.1993, p. 176-179.

Research output: Contribution to journalArticle

Ebihara, T, Hashimoto, T, Kudo, J, Gamou, S, Shimizu, N & Nishikawa, T 1993, 'Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation', Journal of Investigative Dermatology, vol. 100, no. 2, pp. 176-179.
Ebihara T, Hashimoto T, Kudo J, Gamou S, Shimizu N, Nishikawa T. Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation. Journal of Investigative Dermatology. 1993 Feb;100(2):176-179.
Ebihara, Tamotsu ; Hashimoto, Takashi ; Kudo, Jun ; Gamou, Shinobu ; Shimizu, Nobuyoshi ; Nishikawa, Takeji. / Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation. In: Journal of Investigative Dermatology. 1993 ; Vol. 100, No. 2. pp. 176-179.
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