Detection of the high-affinity choline transporter in the MOLT-3 human leukemic T-cell line

Takeshi Fujii, Takashi Okuda, Tatsuya Haga, Koichiro Kawashima

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We previously showed that lymphocytes possess the necessary components to constitute an independent, non-neuronal cholinergic system; these include acetylcholine (ACh) itself, choline acetyltransferase (the ACh-synthesizing enzyme), and both muscarinic and nicotinic ACh receptors (AChRs). In addition, we showed that stimulation of AChRs with their respective agonists elicits a variety of biochemical and functional effects, suggesting that lymphocytic cholinergic system is involved in the regulation of immune function. In nerve terminals, choline taken up via the high-affinity choline transporter (CHT1) is exclusively utilized for ACh synthesis. In the present study, therefore, we investigated the expression of CHT1 in T-lymphocytes. Reverse transcription-polymerase chain reaction analysis revealed that MOLT-3 cells, a human leukemic T-cell line used as a T-lymphocyte model, expressed CHT1 mRNA, but that the CEM and Jurkat T-cell lines did not. Consistent with that finding, specific binding of [3H]hemicholinium-3 (HC-3), an inhibitor of CHT1, and HC-3-sensitive [3H]choline uptake were also detected in MOLT-3 cells. These results suggest that CHT1 plays a role in mediating choline uptake in T-lymphocytes and provides further evidence for the presence of an independent lymphocytic cholinergic system.

Original languageEnglish
Pages (from-to)2131-2134
Number of pages4
JournalLife Sciences
Volume72
Issue number18-19
DOIs
Publication statusPublished - 2003 Mar 28
Externally publishedYes

Fingerprint

T-cells
T-Lymphocytes
Cell Line
Choline
Hemicholinium 3
Cholinergic Agents
Acetylcholine
Jurkat Cells
Choline O-Acetyltransferase
Lymphocytes
Polymerase chain reaction
Nicotinic Receptors
Cholinergic Receptors
Muscarinic Receptors
Transcription
Reverse Transcription
choline transporter
Polymerase Chain Reaction
Messenger RNA
Enzymes

Keywords

  • Acetylcholine
  • High affinity choline transporter
  • T-Lymphocytes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Detection of the high-affinity choline transporter in the MOLT-3 human leukemic T-cell line. / Fujii, Takeshi; Okuda, Takashi; Haga, Tatsuya; Kawashima, Koichiro.

In: Life Sciences, Vol. 72, No. 18-19, 28.03.2003, p. 2131-2134.

Research output: Contribution to journalArticle

Fujii, Takeshi ; Okuda, Takashi ; Haga, Tatsuya ; Kawashima, Koichiro. / Detection of the high-affinity choline transporter in the MOLT-3 human leukemic T-cell line. In: Life Sciences. 2003 ; Vol. 72, No. 18-19. pp. 2131-2134.
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