Detection of Treponema denticola in atherosclerotic lesions

K. Okuda, K. Ishihara, Taneaki Nakagawa, A. Hirayama, Y. Inayama, K. Okuda

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immnnofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.

Original languageEnglish
Pages (from-to)1114-1117
Number of pages4
JournalJournal of Clinical Microbiology
Volume39
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Treponema denticola
Polymerase Chain Reaction
Aorta
Paraffin
Formaldehyde
Immune Sera
Microscopy
Rabbits
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Detection of Treponema denticola in atherosclerotic lesions. / Okuda, K.; Ishihara, K.; Nakagawa, Taneaki; Hirayama, A.; Inayama, Y.; Okuda, K.

In: Journal of Clinical Microbiology, Vol. 39, No. 3, 2001, p. 1114-1117.

Research output: Contribution to journalArticle

Okuda, K, Ishihara, K, Nakagawa, T, Hirayama, A, Inayama, Y & Okuda, K 2001, 'Detection of Treponema denticola in atherosclerotic lesions', Journal of Clinical Microbiology, vol. 39, no. 3, pp. 1114-1117. https://doi.org/10.1128/JCM.39.3.1114-1117.2001
Okuda, K. ; Ishihara, K. ; Nakagawa, Taneaki ; Hirayama, A. ; Inayama, Y. ; Okuda, K. / Detection of Treponema denticola in atherosclerotic lesions. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 3. pp. 1114-1117.
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