Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets

Misako Suzuki, Mitsuru Murata, Yumiko Matsubara, Toshihiro Uchida, Hiroaki Ishihara, Toshiro Shibano, Shin Ichiro Ashida, Kenji Soejima, Yasunori Okada, Yasuo Ikeda

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85 Citations (Scopus)

Abstract

The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.

Original languageEnglish
Pages (from-to)212-216
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume313
Issue number1
DOIs
Publication statusPublished - 2004 Jan 2

Keywords

  • ADAMTS-13
  • Expression
  • Platelets
  • Real-time PCR
  • TTP
  • Thrombosis
  • vWF
  • vWF-CP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Suzuki, M., Murata, M., Matsubara, Y., Uchida, T., Ishihara, H., Shibano, T., Ashida, S. I., Soejima, K., Okada, Y., & Ikeda, Y. (2004). Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets. Biochemical and Biophysical Research Communications, 313(1), 212-216. https://doi.org/10.1016/j.bbrc.2003.11.111